Summary
Isothermal titration calorimetry (ITC) is a useful technique to study RNA-protein interactions as it provides the only method by which the thermodynamic parameters of free energy, enthalpy, and entropy can be directly determined. This chapter presents a general procedure for studying RNA-protein interactions using ITC and gives specific examples for monitoring the binding of Caenorhabditis elegans GLD-1 STAR domain to TGE RNA and the binding of Aquifex aeolicus S6:S18 ribosomal protein heterodimer to an S15-ribosomal RNA complex.
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Acknowledgments
M.I.R. was supported by Research Scholar Grant PF-01-087-01-GMC from the American Cancer Society. S.P.R. was supported by the Damon Runyon Cancer Research Foundation Fellowship (DRG-1723). This research was supported by grants from the Skaggs Institute for Chemical Biology and the National Institutes of Health (NIH, GM53320 and GM53757).
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Recht, M.I., Ryder, S.P., Williamson, J.R. (2008). Monitoring Assembly of Ribonucleoprotein Complexes by Isothermal Titration Calorimetry. In: Lin, RJ. (eds) RNA-Protein Interaction Protocols. Methods in Molecular Biology, vol 488. Humana Press. https://doi.org/10.1007/978-1-60327-475-3_8
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DOI: https://doi.org/10.1007/978-1-60327-475-3_8
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