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Regulation of Convergent Extension by Non-canonical Wnt Signaling in the Xenopus Embryo

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Wnt Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 469))

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Abstract

Non-canonical Wnt signaling is an important regulator of gastrulation in Xenopus. In particular, it has been implicated in the control of convergent extension movements. Convergent extension in the gas-trula occurs primarily in the dorsal tissue of the marginal zone, and explants of this tissue will continue to undergo these movements in isolation. This observation has led to an assay to examine convergent extension movements that is unique to the Xenopus system, and is described herein.

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References

  1. Keller, R., Davidson, L. A., Shook, D. R. (2003) How we are shaped: the biomechanics of gastrulation. Differentiation 71, 171–205.

    Article  PubMed  Google Scholar 

  2. Keller, R. E., Danilchik, M., Gimlich, R., et al. (1985) The function and mechanism of convergent extension during gastrulation of Xenopus laevis. J Embryol Exp Morphol 89 Suppl, 185–209.

    PubMed  Google Scholar 

  3. Shih, J., Keller, R. (1992) Cell motility driving mediolateral intercalation in explants of Xenopus laevis. Development 116, 901–914.

    PubMed  CAS  Google Scholar 

  4. Keller, R., Davidson, L., Edlund, A., et al. (2000) Mechanisms of convergence and extension by cell intercalation. Philos Trans R Soc Lond B Biol Sci 355, 897–922.

    Article  PubMed  CAS  Google Scholar 

  5. Tada, M., Concha, M. L., Heisenberg, C. P. (2002) Non-canonical Wnt signalling and regulation of gastrulation movements. Semin Cell Dev Biol 13, 251–260.

    Article  PubMed  CAS  Google Scholar 

  6. Shulman, J. M., Perrimon, N., Axelrod, J. D. (1998) Frizzled signaling and the developmental control of cell polarity. Trends Genet 14, 452–458.

    Article  PubMed  CAS  Google Scholar 

  7. Boutros, M., Mlodzik, M. (1999) Dishevelled: at the crossroads of divergent intra-cellular signaling pathways. Mech Dev 83, 27–37.

    Article  PubMed  CAS  Google Scholar 

  8. Adler, P. N., Lee, H. (2001) Frizzled signaling and cell-cell interactions in planar polarity. Curr Opin Cell Biol 13, 635–640.

    Article  PubMed  CAS  Google Scholar 

  9. Wallingford, J. B., Fraser, S. E., Harland, R. M. (2002) Convergent extension: the molecular control of polarized cell movement during embryonic development. Dev Cell 2, 695–706.

    Article  PubMed  CAS  Google Scholar 

  10. Axelrod, J. D., Miller, J. R., Shulman, J. M., et al. (1998) Differential recruitment of Dishevelled provides signaling specificity in the planar cell polarity and Wingless signaling pathways. Genes Dev 12, 2610–2622.

    Article  PubMed  CAS  Google Scholar 

  11. Boutros, M., Paricio, N., Strutt, D. I., et al. (1998) Dishevelled activates JNK and discriminates between JNK pathways in planar polarity and wingless signaling. Cell 94, 109–118.

    Article  PubMed  CAS  Google Scholar 

  12. Wallingford, J. B., Rowning, B. A., Vogeli, K. M., et al. (2000) Dishevelled controls cell polarity during Xenopus gastrulation. Nature 405, 81–85.

    Article  PubMed  CAS  Google Scholar 

  13. Tada, M., Smith, J. C. (2000) Xwnt11 is a target of Xenopus Brachyury: regulation of gastrulation movements via Dishevelled, but not through the canonical Wnt pathway. Development 127, 2227–2238.

    PubMed  CAS  Google Scholar 

  14. Keller, R., Tibbetts, P. (1989) Mediolateral cell intercalation in the dorsal, axial meso-derm of Xenopus laevis. Dev Biol 131, 539–549.

    Article  PubMed  CAS  Google Scholar 

  15. Keller, R., Danilchik, M. (1988) Regional expression, pattern and timing of convergence and extension during gastrula-tion of Xenopus laevis. Development 103, 193–209.

    PubMed  CAS  Google Scholar 

  16. Keller, R. E. (1975) Vital dye mapping of the gastrula and neurula of Xenopus laevis. I. Prospective areas and morphogenetic movements of the superficial layer. Dev Biol 42, 222–241.

    Article  PubMed  CAS  Google Scholar 

  17. Keller, R. E. (1976) Vital dye mapping of the gastrula and neurula of Xenopus laevis. II. Prospective areas and morphogenetic movements of the deep layer. Dev Biol 51, 118–137.

    Article  PubMed  CAS  Google Scholar 

  18. Doniach, T., Phillips, C. R., Gerhart, J. C. (1992) Planar induction of anteroposterior pattern in the developing central nervous system of Xenopus laevis. Science 257, 542–545.

    Article  PubMed  CAS  Google Scholar 

  19. Sokol, S. Y. (1996) Analysis of Dishevelled signalling pathways during Xenopus development. Curr Biol 6, 1456–1467.

    Article  PubMed  CAS  Google Scholar 

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Petersen, L.F., Ninomiya, H., Winklbauer, R. (2008). Regulation of Convergent Extension by Non-canonical Wnt Signaling in the Xenopus Embryo. In: Vincan, E. (eds) Wnt Signaling. Methods in Molecular Biology, vol 469. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-469-2_30

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  • DOI: https://doi.org/10.1007/978-1-60327-469-2_30

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-468-5

  • Online ISBN: 978-1-60327-469-2

  • eBook Packages: Springer Protocols

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