Abstract
Atomic force microscopy (AFM) can directly visualize single molecules in solution, which makes it an extremely powerful technique for carrying out studies of biological complexes and the processes in which they are involved. A recent development, called Recognition Imaging, allows the identification of a specific type of protein in solution AFM images, a capability that greatly enhances the power of the AFM approach for studies of complex biological materials. In this technique, an antibody against the protein of interest is attached to an AFM tip. Scanning a sample with this tip generates a typical topographic image simultaneously and in exact spatial registration with a “recognition image.” The latter identifies the locations of antibody-antigen binding events and thus the locations of the protein of interest in the image field. The recognition image can be electronically superimposed on the topographic image, providing a very accurate map of specific protein locations in the topographic image. This technique has been mainly used in in vitro studies of biological complexes and reconstituted chromatin, but has great potential for studying chromatin and protein complexes isolated from nuclei.
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Acknowledgments
Support from the National Institutes of Health is gratefully acknowledged. Also, Ralph Bash died in October 2007; this chapter is dedicated to our longtime colleague and coworker whose skills in AFM analysis of chromatin contributed immeasurably to the development of these approaches.
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Lohr, D., Wang, H., Bash, R., Lindsay, S.M. (2008). Recognition Imaging of Chromatin and Chromatin-Remodeling Complexes in the Atomic Force Microscope. In: Hancock, R. (eds) The Nucleus. Methods in Molecular Biology, vol 464. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-461-6_8
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DOI: https://doi.org/10.1007/978-1-60327-461-6_8
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