Abstract
Electrospray mass spectrometry can be used to detect ligand-DNA noncovalent complexes formed in solution. This chapter describes how to determine equilibrium association constants of the complexes. Particular attention is devoted to describing how to tune an electrospray mass spectrometer using a 12-mer oligodeoxynucleotides duplex in order to perform these experiments. This protocol can then be applied to any nucleic acid structure that can be ionized with electrospray mass spectrometry.
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Acknowledgments
I would like to thank Frédéric Rosu, Nicolas Smargiasso, Joëlle Widart, Dominique Baiwir, and Edwin De Pauw for their comments on the manuscript. The Fonds de la Recherche Scientifique-FNRS and the University of Liège are gratefully acknowledged for financial support. The author is a FNRS research associate.
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Gabelica, V. (2010). Determination of Equilibrium Association Constants of Ligand–DNA Complexes by Electrospray Mass Spectrometry. In: Fox, K. (eds) Drug-DNA Interaction Protocols. Methods in Molecular Biology, vol 613. Humana Press. https://doi.org/10.1007/978-1-60327-418-0_6
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DOI: https://doi.org/10.1007/978-1-60327-418-0_6
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