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Drug Binding to DNA⋅RNA Hybrid Structures

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Drug-DNA Interaction Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 613))

Abstract

DNA·RNA hybrid duplexes are functionally important structures in gene expression that are underutilized as potential drug targets. Several tools are described here for the discovery and characterization of small molecules capable of the selective recognition of DNA·RNA hybrid structures. Competition dialysis and thermal denaturation of mixtures of polynucleotide structures can be used to identify small molecules that bind selectively to DNA·RNA hybrids. An assay that measures small molecule inhibition of RNase H can be used to measure a functional response to these ligands.

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Acknowledgments

This work supported by award number R01GM077422 from the National Institute of General Medical Sciences. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIGMS or NIH.

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Authors and Affiliations

  1. School of Pharmacy, University of Bradford, Bradford, West Yorkshire, UK

    Richard T. Wheelhouse

  2. James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA

    Jonathan B. Chaires

Authors
  1. Richard T. Wheelhouse
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  2. Jonathan B. Chaires
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Corresponding author

Correspondence to Jonathan B. Chaires .

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Editors and Affiliations

  1. School of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, United Kingdom

    Keith R. Fox

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Wheelhouse, R.T., Chaires, J.B. (2010). Drug Binding to DNA⋅RNA Hybrid Structures. In: Fox, K. (eds) Drug-DNA Interaction Protocols. Methods in Molecular Biology, vol 613. Humana Press. https://doi.org/10.1007/978-1-60327-418-0_4

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  • DOI: https://doi.org/10.1007/978-1-60327-418-0_4

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-417-3

  • Online ISBN: 978-1-60327-418-0

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