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Native Techniques for Analysis of Mitochondrial Protein Import

  • F. -Nora Vögtle
  • Oliver Schmidt
  • Agnieszka Chacinska
  • Nikolaus Pfanner
  • Chris Meisinger
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 619)

Abstract

During the evolution of eukaryotic cells, the majority of mitochondrial genes have been transferred to the nuclear genome with the consequence that most mitochondrial proteins have to be imported into the organelle. This process occurs usually in a post-translational manner. In order to accomplish this task elaborate protein machineries have evolved that import precursor proteins in a concerted fashion and sort them into the four mitochondrial subcompartments. Native techniques such as Blue Native Electrophoresis allow to analyze the composition of the import machineries and to characterize the cooperation of import components. The analysis has led to the discovery of new components and import pathways of mitochondria.

Key words

Mitochondria protein import translocation intermediates Blue Native PAGE protein complex analysis 

Notes

Acknowledgments

This work was supported by the Trinational Research Training Group GRK 1478, the Sonderforschungsbereich 746, the Excellence Initiative of the German Federal and State Government (EXC 294) and the Gottfried Wilhelm Leibniz Program.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • F. -Nora Vögtle
    • 1
  • Oliver Schmidt
    • 1
  • Agnieszka Chacinska
    • 1
  • Nikolaus Pfanner
    • 1
  • Chris Meisinger
    • 1
  1. 1.Institut für Biochemie und Molekularbiologie, ZBMZ, and Centre for Biological Signalling Studies (bioss), Universität Freiburg, FreiburgFreiburgGermany

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