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Flow Cytometric Studies on Intracellular Drug Fluorescence

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Techniques in Cell Cycle Analysis

Part of the book series: Biological Methods ((BM))

Abstract

Flow cytometry has rapidly evolved from a technique for detecting and sorting cells on the basis of their DNA content or immunological markers into a useful tool for detection and quantitation of intracellular drug fluorescence (2,25,30,37). Recent studies have shown that intracellular content of fluorescent drugs can be rapidly quantitated on a cell-to-cell basis by this sophisticated analytical method. Thus, one can analyze intracellular drug transport (influx, efflux), retention, and/or binding, and correlate these parameters with effects on cellular metabolism and proliferation (21,22).

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Authors and Affiliations

  1. Comprehensive Cancer Center and Department of Oncology, University of Miami Medical School, Miami, Florida

    Awtar Krishan

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  1. Awtar Krishan
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Joe W. Gray Zbigniew Darzynkiewicz

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© 1987 The Humana Press Inc.

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Krishan, A. (1987). Flow Cytometric Studies on Intracellular Drug Fluorescence. In: Gray, J.W., Darzynkiewicz, Z. (eds) Techniques in Cell Cycle Analysis. Biological Methods. Humana Press. https://doi.org/10.1007/978-1-60327-406-7_11

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  • DOI: https://doi.org/10.1007/978-1-60327-406-7_11

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-097-8

  • Online ISBN: 978-1-60327-406-7

  • eBook Packages: Springer Book Archive

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