Abstract
This experiment introduces the student to two commonly used immunoelectrophoretic techniques; rocket immunoelectrophoresis, also known as electroimmunoassay, and 2-D immunoelectrophoresis.
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References
Laurell, C-B. (1966) Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal. Biochem. 15, 45–52.
Minchin Clarke, H.G. and Freeman, T. (1968) Quantitative immunoelectrophoresis of human serum proteins. Clin. Sci. 35, 403–413.
Dako Information Sheet No. 4. Provided by DAKO-immunoglobulins a/s, Copenhagen, Denmark (or local supplier).
Further Reading
A Manual of Quantitative Immunoelectrophoresis-Methods and Applications (1973) Scand. J. Immunol 2,(Suppl. 1) (Axelsen, N.H., Kroll, J., and Week, B., eds.) Universitatsforgleget, Oslo.
Handbook of Immunoprecipitation-in-Gel Techniques (1983) (Axelsen, N.H., Ed.) Blackwell Scientific Publications.
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© 1986 The Humana Press Inc.
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Walker, J.M. (1986). Immunoelectrophoretic Techniques. In: Slater, R.J. (eds) Experiments in Molecular Biology. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-405-0_21
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DOI: https://doi.org/10.1007/978-1-60327-405-0_21
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-082-4
Online ISBN: 978-1-60327-405-0
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