Abstract
Living cells contain two forms of nucleic acid-deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). It has been known for a number of years that RNA molecules are involved in the expression of the genetic material (DNA) and are, in fact, the initial products of gene expression that go on to direct protein synthesis. Knowledge of the structure, metabolism, and mode of action of RNA in protein synthesis is therefore fundamental to our understanding of the control of gene expression, cell division, growth, and development. Furthermore, the recent spectacular advances in genetic engineering depend on a working knowledge of RNA synthesis and its control.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Littauer, U.Z. and Soreq, H. (1982) The Regulatory Function of Poly(A) and Adjacent 3′ Sequences in Translated RNA, in Progress in Nucleic Acid Research and Molecular Biology Vol. 27 (Coin, W.E., ed.) Academic Press, London and New York.
Aviv, H. and Leder, P. (1972) Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid cellulose. Proc. Natl. Acad. Sci. USA 69, 1408–1412.
Grierson, D. and Speirs, J. (1983) Protein Synthesis In Vitro, in Techniques in Molecular Biology. (Walker, J.M. and Gaastra, W., eds.). Croom Helm, London and Canberra, and Macmillan, New York.
Williams, J.S. (1981) The Preparation and Screening of a cDNA Clone Bank, in Genetic Engineering (Williamson, R., ed.). Vol 1., Academic Press, New York.
Taylor, J.M. (1979) The isolation of eukaryotic messenger RNA. Ann. Rev. Biochem. 48, 681–717.
Slater, R.J. (1983) The Extraction and Fractionation of RNA, in Techniques in Molecular Biology. (Walker, J.M. and Gaastra, W., Eds.). Croom Helm, London and Canberra, and Macmillan, New York.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1986 The Humana Press Inc.
About this protocol
Cite this protocol
Slater, R.J. (1986). The Extraction and Affinity Chromatography of Messenger RNA. In: Slater, R.J. (eds) Experiments in Molecular Biology. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-405-0_12
Download citation
DOI: https://doi.org/10.1007/978-1-60327-405-0_12
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-082-4
Online ISBN: 978-1-60327-405-0
eBook Packages: Springer Book Archive