Abstract
Arrays of immobilized antimicrobial peptides are used to detect bacterial, viral, and rickettsial pathogens, including inactivated biothreat agents. These arrays differ from the many combinatorial peptide arrays described in the literature in that the peptides used here have naturally evolved to interact with and disrupt microbial membranes with high affinity but broad specificity. The interaction of these naturally occurring peptides with membranes of pathogens has been harnessed for the purpose of detection, with immobilized antimicrobial peptides acting as “capture” molecules in detection assays. Methods are presented for immobilizing the antimicrobial peptides in planar arrays, performing direct and sandwich assays, and detecting bound targets.
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Acknowledgements
The authors would like to acknowledge the financial support of Joint Science and Technology Office for Chemical and Biological Defense/Defense Threat Reduction Agency (#AA04DET005, 8.10016_08_NRL_B) and the National Institutes of Health (R01 EB00680). NVK was a recipient of a National Research Council fellowship. The views expressed herein are those of the authors and do not represent those of the US Navy, the US Department of Defense, or the US government.
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Taitt, C.R., North, S.H., Kulagina, N.V. (2009). Antimicrobial Peptide Arrays for Detection of Inactivated Biothreat Agents. In: Cretich, M., Chiari, M. (eds) Peptide Microarrays. Methods in Molecular Biology™, vol 570. Humana Press. https://doi.org/10.1007/978-1-60327-394-7_11
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DOI: https://doi.org/10.1007/978-1-60327-394-7_11
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