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Exploring and Profiling Protein Function with Peptide Arrays

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Peptide Microarrays

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 570))

Abstract

Development of array technologies started in the late 1980s and was first extensively applied to DNA arrays especially in the genomic field. Today this technique has become a powerful tool for high-throughput approaches in biology and chemistry. Progresses were mainly driven by the human genome project and were associated with the development of several new technologies, which led to the onset of additional “omic” topics like proteomics, glycomics, antibodyomics or lipidomics. The main characteristics of the array technology are (i) spatially addressable immobilization of a huge number of different capture molecules; (ii) probing the array in a simultaneous and highly parallel manner with a biological sample; (iii) tendency towards miniaturization of the arrays; and (iv) software-supported read-out and data analysis. We review some general concepts about peptide arrays on planar supports and point out technical aspects concerning the generation of peptide microarrays. Finally, we discuss recent applications by describing relevant literature.

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Acknowledgments

Financial support from Detsche Froschungsgemeinschaft (SFB449) is gratefully acknowledged.

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Authors and Affiliations

  1. Institut für Medizinische Immunologie, Charité-Universitätsmedizin Berlin, Berlin, Germany

    Victor E. Tapia, Bernhard Ay & Rudolf Volkmer

Authors
  1. Victor E. Tapia
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  2. Bernhard Ay
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  3. Rudolf Volkmer
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Editor information

Editors and Affiliations

  1. Inst. Chimica del Riconoscimento, CNR, Via Mario Bianco 9, Milano, 20131, Italy

    Marina Cretich

  2. Inst. Chimica del Riconoscimento, CNR, Via Mario Bianco 9, Milano, 20131, Italy

    Marcella Chiari

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Tapia, V.E., Ay, B., Volkmer, R. (2009). Exploring and Profiling Protein Function with Peptide Arrays. In: Cretich, M., Chiari, M. (eds) Peptide Microarrays. Methods in Molecular Biology™, vol 570. Humana Press. https://doi.org/10.1007/978-1-60327-394-7_1

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  • DOI: https://doi.org/10.1007/978-1-60327-394-7_1

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-393-0

  • Online ISBN: 978-1-60327-394-7

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