Abstract
Chromatin immunoprecipitation (ChIP) is a well-established procedure for protein−DNA interaction research. ChIP-chip, combining chromatin immunoprecipitation (ChIP) and microarray technology (Chip), enables scientists to survey genome-wide DNA binding sites for a given protein. The ChIP-chip technique has been used to identify transcription factor binding sites, explore epigenomic information and investigate factors in DNA replicate/repairs. Here we describe a protocol for ChIP-chip to study Pituitary Tumor Transforming Gene (PTTG1) in mammalian cells.
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Acknowledgments
This work was supported by NIH Grant CA 75979 (SM), T32 DK007770, and The Doris Factor Molecular Endocrinology Laboratory. We thank Dr. Shlomo Melmed for his critical discussion in preparing this protocol.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Tong, Y., Falk, J. (2009). Genome-Wide Analysis for Protein−DNA Interaction: ChIP-Chip. In: Park-Sarge, OK., Curry, T. (eds) Molecular Endocrinology. Methods in Molecular Biology, vol 590. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-378-7_15
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DOI: https://doi.org/10.1007/978-1-60327-378-7_15
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