Abstract
Characterization of the binding of a hormone to its cognate receptor is a cornerstone of many studies in molecular and cellular endocrinology since this event represents the beginning of a specific cellular response, generally from a highly regulated extracellular messenger. The premise of hormone−receptor interaction follows from the law of mass action describing a reversible second-order reaction, hormone plus receptor, to give a non-covalently associated hormone-receptor complex. From this basic principle, a host of useful experimental parameters are available to the interested investigator. This chapter is focused on development of the experimental and mathematical underpinning of hormone−receptor interaction, with emphasis on a gonadotropin, chorionic gonadotropin (or luteinizing hormone), binding to the luteinizing hormone receptor, a member of the G protein-coupled receptor family. The general concepts and approaches developed herein are, however, valid to most interacting systems.
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Acknowledgments
This chapter is dedicated to the memory of Yongsheng Li who contributed significantly to research on gonadotropins and their receptors. It is a pleasure to thank Geneva DeMars for helpful comments and assistance. Supported by NIH research grants DK33973 and DK69711.
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Puett, D., Angelova, K. (2009). Determining the Affinity of Hormone−Receptor Interaction. In: Park-Sarge, OK., Curry, T. (eds) Molecular Endocrinology. Methods in Molecular Biology, vol 590. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-378-7_1
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DOI: https://doi.org/10.1007/978-1-60327-378-7_1
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