Abstract
Cationic liposomes composed of 3β-[N-(N´ N´–dimethylaminoethane)carbamoyl] cholesterol (DC–Chol) and dioleoylphosphatidylethanolamine (DOPE) (DC–Chol/DOPE liposome, molar ratio, 1:1 or 3:2) prepared by the dry-film method have been often used as non-viral gene delivery vectors. We have shown that a more efficient transfection in medium with serum was achieved using DC–Chol/DOPE liposomes (molar ratio, 1:2) than those (3:2), and preparation method by a modified ethanol injection than the dry-film. The most efficient DC–Chol/DOPE liposome for gene transfer was molar ratio (1:2) and prepared by a modified ethanol injection method. The enhanced transfection is related to an increase in the release of DNA in the cytoplasm by the large lipoplex during incubation in opti-MEM I reduced-serum medium (optiMEM), not to an increased cellular association with the lipoplex. Cationic liposomes rich in DOPE prepared by a modified ethanol injection method will help to improve the efficacy of liposome vector systems for gene delivery.
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Maitani, Y. (2010). Lipoplex Formation Using Liposomes Prepared by Ethanol Injection. In: Weissig, V. (eds) Liposomes. Methods in Molecular Biology, vol 605. Humana Press. https://doi.org/10.1007/978-1-60327-360-2_27
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DOI: https://doi.org/10.1007/978-1-60327-360-2_27
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