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Nanoliposomes: Preparation and Analysis

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Liposomes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 605))

Abstract

Nanoliposome, or submicron bilayer lipid vesicle, is a new technology for the encapsulation and delivery of bioactive agents. The list of bioactive material that can be incorporated to nanoliposomes is immense, ranging from pharmaceuticals to cosmetics and nutraceuticals. Because of their biocompatibility and biodegradability, along with their nanosize, nanoliposomes have potential applications in a vast range of fields, including nanotherapy (e.g. diagnosis, cancer therapy, gene delivery), cosmetics, food technology and agriculture. Nanoliposomes are able to enhance the performance of bioactive agents by improving their solubility and bioavailability, in vitro and in vivo stability, as well as preventing their unwanted interactions with other molecules. Another advantage of nanoliposomes is cell-specific targeting, which is a prerequisite to attain drug concentrations required for optimum therapeutic efficacy in the target site while minimising adverse effects on healthy cells and tissues. This chapter covers nanoliposomes, particularly with respect to their properties, preparation methods and analysis.

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Abbreviations

Chol:

Cholesterol

DCP:

Dicetylphosphate

DPPC:

Dipalmitoyl phosphatidylcholine

EE:

Entrapment efficiency

FFF:

Field flow fractionation

HM-liposomes:

Lipid vesicles prepared by the heating method

HPLC:

High-performance liquid chromatography

OH:

Hydroxyl group

LUV:

Large unilamellar vesicles

MLV:

Multilamellar vesicles

NMR:

Nuclear magnetic resonance

PC:

Phosphatidylcholine

SPM:

Scanning Probe Microscopy

SUV:

Small unilamellar vesicles

T c :

Phase transition temperature

TLC:

Thin layer chromatography

T m :

Melting temperature

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Author information

Authors and Affiliations

  1. Phosphagenics R&D Laboratory, Clayton, VIC, Australia

    M. R. Mozafari

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  1. M. R. Mozafari
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Correspondence to M. R. Mozafari .

Editor information

Editors and Affiliations

  1. College of Pharmacy, Midwestern University, N. 59th Avenue 19555, Glendale, 85308, U.S.A.

    Volkmar Weissig

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Mozafari, M.R. (2010). Nanoliposomes: Preparation and Analysis. In: Weissig, V. (eds) Liposomes. Methods in Molecular Biology, vol 605. Humana Press. https://doi.org/10.1007/978-1-60327-360-2_2

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  • DOI: https://doi.org/10.1007/978-1-60327-360-2_2

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-359-6

  • Online ISBN: 978-1-60327-360-2

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