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Real-Time Quantitative PCR, Pathogen Detection and MIQE

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PCR Detection of Microbial Pathogens

Part of the book series: Methods in Molecular Biology ((MIMB,volume 943))

Abstract

Nucleic acids are the ultimate biomarker and real-time PCR (qPCR) is firmly established as the method of choice for nucleic acid detection. Together, they allow the accurate, sensitive and specific identification of pathogens, and the use of qPCR has become routine in diagnostic laboratories. The reliability of qPCR-based assays relies on a combination of optimal sample selection, assay design and validation as well as appropriate data analysis and the “Minimal Information for the Publication of real-time PCR” (MIQE) guidelines aim to improve both the reliability of assay design as well as the transparency of reporting, essential conditions if qPCR is to remain the benchmark technology for molecular diagnosis.

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Author information

Authors and Affiliations

  1. Blizard Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK

    Gemma Johnson & Stephen A. Bustin

  2. Sigma-Aldrich, Haverhill, UK

    Tania Nolan

Authors
  1. Gemma Johnson
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  2. Tania Nolan
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  3. Stephen A. Bustin
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Corresponding author

Correspondence to Stephen A. Bustin .

Editor information

Editors and Affiliations

  1. Deptartment of Microbiology, St. Bartholomew's Hospital, Newark Street 80, London, E1 2ES, United Kingdom

    Mark Wilks

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Johnson, G., Nolan, T., Bustin, S.A. (2013). Real-Time Quantitative PCR, Pathogen Detection and MIQE. In: Wilks, M. (eds) PCR Detection of Microbial Pathogens. Methods in Molecular Biology, vol 943. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-353-4_1

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  • DOI: https://doi.org/10.1007/978-1-60327-353-4_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-352-7

  • Online ISBN: 978-1-60327-353-4

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