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Quantification of Endogenous Retinoids

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Retinoids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 652))

Abstract

Numerous physiological processes require retinoids, including development, nervous system function, immune responsiveness, proliferation, differentiation, and all aspects of reproduction. Reliable retinoid quantification requires suitable handling and, in some cases, resolution of geometric isomers that have different biological activities. Here we describe procedures for reliable and accurate quantification of retinoids, including detailed descriptions for handling retinoids, preparing standard solutions, collecting samples and harvesting tissues, extracting samples, resolving isomers, and detecting with high sensitivity. Sample-specific strategies are provided for optimizing quantification. Approaches to evaluate assay performance also are provided. Retinoid assays described here for mice also are applicable to other organisms including zebrafish, rat, rabbit, and human and for cells in culture. Retinoid quantification, especially that of retinoic acid, should provide insight into many diseases, including Alzheimer’s disease, type 2 diabetes, obesity, and cancer.

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Author information

Authors and Affiliations

  1. Department of Pharmaceutical Sciences, University of Maryland, Baltimore, MD, USA

    Maureen A. Kane

  2. Department of Nutritional Science and Toxicology, University of California, Berkeley, CA, USA

    Joseph L. Napoli

Authors
  1. Maureen A. Kane
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  2. Joseph L. Napoli
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Editor information

Editors and Affiliations

  1. David Geffen School of Medicine, Dept. Physiology, University of California, Los Angeles, Charles Young Dr. S. 650, Los Angeles, 90095-1751, California, USA

    Hui Sun

  2. , Jules Stein Eye Institute, UCLA School of Medicine, 100 Stein Plaza, BH-667, Los Angeles, 90095, California, USA

    Gabriel H. Travis

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Kane, M.A., Napoli, J.L. (2010). Quantification of Endogenous Retinoids. In: Sun, H., Travis, G. (eds) Retinoids. Methods in Molecular Biology, vol 652. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-325-1_1

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  • DOI: https://doi.org/10.1007/978-1-60327-325-1_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-324-4

  • Online ISBN: 978-1-60327-325-1

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