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Imaging Vasculature and Lymphatic Flow in Mice Using Quantum Dots

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Bioluminescence

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 574))

Abstract

Quantum dots are ideal probes for fluorescent imaging of vascular and lymphatic tissues. On injection into appropriate sites, red- and near-infrared-emitting quantum dots provide excellent definition of vasculature, lymphoid organs, and lymph nodes draining both normal tissues and tumors. We detail methods for use with commercially available quantum dots and discuss common difficulties.

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Acknowledgments

The authors wish to acknowledge financial support from the NIH BRP program under grant number EB00364. MB also wishes to acknowledge Carnegie Mellon University for faculty start-up funds.

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Authors and Affiliations

  1. Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, PA, USA

    Byron Ballou, Lauren A. Ernst, Susan Andreko, James A. J. Fitzpatrick, Alan S. Waggoner & Marcel P. Bruchez

  2. MEMPHYS, University of Southern Denmark, Odense, Denmark

    B. Christoffer Lagerholm

  3. Department of Biology, Carnegie Mellon University, Pittsburgh, PA, USA

    Alan S. Waggoner

  4. Department of Chemistry, Carnegie Mellon University, Pittsburgh, PA, USA

    Marcel P. Bruchez

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  1. Byron Ballou
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  2. Lauren A. Ernst
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  3. Susan Andreko
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  4. James A. J. Fitzpatrick
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  5. B. Christoffer Lagerholm
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  6. Alan S. Waggoner
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  7. Marcel P. Bruchez
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Editor information

Editors and Affiliations

  1. Dept. Surgery, University of North Carolina, Chapel Hill, 27599-7228, U.S.A.

    Preston B. Rich

  2. Dept. Surgery, University of North Carolina, Manning Drive 90, Chapel Hill, 27599-7161, U.S.A.

    Christelle Douillet

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Ballou, B. et al. (2009). Imaging Vasculature and Lymphatic Flow in Mice Using Quantum Dots. In: Rich, P., Douillet, C. (eds) Bioluminescence. Methods in Molecular Biology™, vol 574. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-321-3_6

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  • DOI: https://doi.org/10.1007/978-1-60327-321-3_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-320-6

  • Online ISBN: 978-1-60327-321-3

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