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Imaging Vasculature and Lymphatic Flow in Mice Using Quantum Dots

  • Byron Ballou
  • Lauren A. Ernst
  • Susan Andreko
  • James A. J. Fitzpatrick
  • B. Christoffer Lagerholm
  • Alan S. Waggoner
  • Marcel P. Bruchez
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 574)

Abstract

Quantum dots are ideal probes for fluorescent imaging of vascular and lymphatic tissues. On injection into appropriate sites, red- and near-infrared-emitting quantum dots provide excellent definition of vasculature, lymphoid organs, and lymph nodes draining both normal tissues and tumors. We detail methods for use with commercially available quantum dots and discuss common difficulties.

Key words

Quantum dots in vivo animals vasculature circulation lymph nodes sentinel lymph nodes lymphatic vessels 

Notes

Acknowledgments

The authors wish to acknowledge financial support from the NIH BRP program under grant number EB00364. MB also wishes to acknowledge Carnegie Mellon University for faculty start-up funds.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Byron Ballou
    • 1
  • Lauren A. Ernst
    • 1
  • Susan Andreko
    • 1
  • James A. J. Fitzpatrick
    • 1
  • B. Christoffer Lagerholm
    • 2
  • Alan S. Waggoner
    • 1
    • 3
  • Marcel P. Bruchez
    • 1
    • 4
  1. 1.Molecular Biosensor and Imaging Center, Carnegie Mellon UniversityPittsburghUSA
  2. 2.MEMPHYS, University of Southern DenmarkOdenseDenmark
  3. 3.Department of BiologyCarnegie Mellon UniversityPittsburghUSA
  4. 4.Department of ChemistryCarnegie Mellon UniversityPittsburghUSA

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