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Fluorescence Imaging of Tumors with “Smart” pH-Activatable Targeted Probes

  • Daisuke Asanuma
  • Hisataka Kobayashi
  • Tetsuo Nagano
  • Yasuteru Urano
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 574)

Abstract

One goal of molecular imaging is to establish a widely applicable technique for specific detection of tumors with minimal background originated from non-target tissues. In this study, a “smart” activatable strategy for specific tumor imaging is proposed in which pH-activatable targeted probes specifically detect tumors after binding to the target cell surface proteins, internalization, and eventual acidic pH activation within the acidic organelles. We successfully visualized submillimeter-sized tumors using this strategy in two different tumor mouse models. Since the design of pH-activatable targeted probes can be applied to any target molecules on the cell surface that are to be internalized after ligand binding, this imaging strategy can afford a general and powerful method to diagnose and monitor the target tumors.

Keywords

Optical tumor imaging fluorescence molecular probes pH receptor-mediated endocytosis 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Daisuke Asanuma
    • 1
  • Hisataka Kobayashi
    • 2
  • Tetsuo Nagano
    • 1
  • Yasuteru Urano
    • 2
  1. 1.Graduate School of Pharmaceutical Sciences, The University of TokyoTokyoJapan
  2. 2.Molecular Imaging Program, National Cancer Institute, National Institute of Health, Center for Cancer ResearchBethesdaUSA

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