Summary
The most frequently used approach to produce single-chain Fv fragments (scFv) and Fab in Escherichia coli is to express them in the periplasm of the bacteria. We present here an alternative procedure that uses cytoplasmic expression of soluble active scFv. This can be accomplished by using either specially engineered E. coli strains or hyperstable scFvs.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Guglielmi, L., Martineau, P. (2009). Expression of Single-Chain Fv Fragments in E. coli Cytoplasm. In: Aitken, R. (eds) Antibody Phage Display. Methods in Molecular Biology, vol 562. Humana Press. https://doi.org/10.1007/978-1-60327-302-2_17
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DOI: https://doi.org/10.1007/978-1-60327-302-2_17
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