Abstract
The need for large-scale collection of rodent embryos and individual embryonic tissues for genomic and proteomic studies requires modification of traditional practices of embryo necropsy. The sample intended for transcriptome study should be rapidly dissected and stabilized to preserve its molecular integrity. The retrieval of high-quality RNA, DNA, and proteins from the target tissue is crucial for informative molecular analysis (e.g., gene profiling on microarray platform). We present a reliable method of collection and preparation of rodent embryos for genomic studies supported by detailed protocols and RNA extraction results for different stages of mouse embryonic development.
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Acknowledgments
The authors would like to thank Anna Trivet of NCI-Frederick for her technical expertise and Keith Rogers, currently of BSF–Singapore for his continuous support during many different phases of this study, also Scott Lawrence of NCI-FCRDC, DCTD, DTP, PADIS for generous help with photography and Tamara Morgan of Histotechnology Laboratory, NCI–Frederick for technical assistance. This work was funded by NCI Contract HHSN261200800001E.
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Golubeva, Y., Symer, D. (2014). Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study. In: Lewandoski, M. (eds) Mouse Molecular Embryology. Methods in Molecular Biology, vol 1092. Humana Press, Boston, MA. https://doi.org/10.1007/978-1-60327-292-6_20
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DOI: https://doi.org/10.1007/978-1-60327-292-6_20
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