Abstract
Gene transfer techniques such as electroporation and lipofection are powerful systems for investigating gene function. In this chapter we focus on the methods and applications of gene transfer into specific cells and tissues of post-implantation mouse embryos.
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References
Sugar IP, Neumann E (1984) Stochastic model for electric field-induced membrane pores. Electroporation. Biophys Chem 19:211–225
Felgner PL, Gadek TR, Holm M, Roman R, Chan HW, Wenz M, Northrop JP, Ringold GM, Danielsen M (1987) Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc Natl Acad Sci U S A 84:7413–7417
Mellitzer G, Hallonet M, Chen L, Ang SL (2002) Spatial and temporal ‘knock down’ of gene expression by electroporation of double-stranded RNA and morpholinos into early postimplantation mouse embryos. Mech Dev 118:57–63
Davidson BP, Tsang TE, Khoo PL, Gad JM, Tam PP (2003) Introduction of cell markers into germ layer tissues of the mouse gastrula by whole embryo electroporation. Genesis 35:57–62
Osumi N, Inoue T (2001) Gene transfer into cultured mammalian embryos by electroporation. Methods 24:35–42
Mizushima S, Nagata S (1990) pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res 18:5322
Swartz M, Eberhart J, Mastick GS, Krull CE (2001) Sparking new frontiers: using in vivo electroporation for genetic manipulations. Dev Biol 233:13–21
Sheehy NT, Cordes KR, White MP, Ivey KN, Srivastava D (2010) The neural crest-enriched microRNA miR-452 regulates epithelial-mesenchymal signaling in the first pharyngeal arch. Development 137:4307–4316
Nagy A, Gertsenstein M, Vintersten K, Behringer RR (2003) Manipulating the mouse embryo. Cold Spring Harbor Laboratory, Cold Spring Harbor
Fukuchi-Shimogori T, Grove EA (2001) Neocortex patterning by the secreted signaling molecule FGF8. Science 294:1071–1074
Saito T, Nakatsuji N (2001) Efficient gene transfer into the embryonic mouse brain using in vivo electroporation. Dev Biol 240:237–246
Saito T (2006) In vivo electroporation in the embryonic mouse central nervous system. Nat Protoc 1:1552–1558
Moore-Scott BA, Gordon J, Blackburn CC, Condie BG, Manley NR (2003) New serum-free in vitro culture technique for midgestation mouse embryos. Genesis 35:164–168
Acknowledgements
Work in the Trainor laboratory is supported by the Stowers Institute for Medical Research, the March of Dimes (#6-FY08-265), and the National Institute for Dental and Craniofacial Research (DE016082).
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Sakai, D., Trainor, P.A. (2014). Gene Transfer Techniques in Whole Embryo Cultured Post-implantation Mouse Embryos. In: Lewandoski, M. (eds) Mouse Molecular Embryology. Methods in Molecular Biology, vol 1092. Humana Press, Boston, MA. https://doi.org/10.1007/978-1-60327-292-6_15
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DOI: https://doi.org/10.1007/978-1-60327-292-6_15
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Publisher Name: Humana Press, Boston, MA
Print ISBN: 978-1-60327-290-2
Online ISBN: 978-1-60327-292-6
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