Skip to main content
SpringerLink
Log in
Book cover

Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants pp 313–324Cite as

Spearmint Plantlet Culture System as a Means to Study Secondary Metabolism

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 547))

Summary

Spearmint has one major monoterpene, (−)-carvone, that constitutes up to 90% of all the monoterpenes present. Likewise, the major phenylpropanoid—rosmarinic acid—in spearmint accounts for up to 70% of the phenylpropanoids produced from the plant. These two compounds are each produced by separate distinct biosynthetic pathways which provide an excellent opportunity to study the influence of a wide number of environmental and chemical conditions on secondary metabolism and plant growth. The techniques presented in this chapter employ 1 g of fresh weight material for each secondary metabolite analyses. Analysis of single compounds obtained from the two distinct metabolic pathways simplifies the interpretation of the metabolic results allowing for direct correlations of culture factors on secondary metabolism.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Croteau, R. T., Kutchan, M., and Lewis, N. G. (2000). Natural products (secondary metabolites), in Biochemistry & Molecular Biology of Plants (Buchan, B., Gruissem, W. and Jones, R., eds.), American Society of Plant Physiologists, Rockville, MD, pp.1250–1318.

    Google Scholar 

  2. Hegnauer, R. (1953). The content of essential oil and carvone in various species of mint. Ber. Schweiz. Bot. Ges. 63, 90–102.

    CAS  Google Scholar 

  3. Sticher, V. O., and Fluck, H. (1968). Composition of genuine extracted and distilled essential oils of some Mentha species. Pharmaceutica Acta Helvetica 43, 411.

    CAS  Google Scholar 

  4. Bourweig, D., and Pohl, R. (1973). Flavonoids from Mentha longifolia<. Planta Medica 24, 304–314.

    Article  Google Scholar 

  5. Inoye, T., Sugimoto, Y., Masuda, H., and Kamei, C. (2002). Antiallergic effect of flavonoid glycosides obtained from Mentha piperita L. Biol. Pharm. Bull. 25, 256–259.

    Article  Google Scholar 

  6. Tisserat, B., and Vaughn, S. F. (2001). Essential oils enhanced by ultra-high carbon dioxide levels from Lamiaceae species grown in vitro< and in vivo<. Plant Cell Rep. 20, 361–368.

    Article  CAS  Google Scholar 

  7. Tisserat, B., Vaughn, S. F., and Silman, R. (2002). Influence of modified oxygen and carbon dioxide atmospheres on mint and thyme plant growth, morphogenesis and secondary metabolism in vitro. Plant Cell Rep. 20, 912–916.

    Article  CAS  Google Scholar 

  8. Yang, R., and Shetty, K. (1998). Stimulation of rosmarinic acid in shoot cultures or oregano (Origanum vulgare<) clonal line in response to proline, proline analog and proline precursors. J. Agric. Food Chem. 46, 2888–2893.

    Article  CAS  Google Scholar 

  9. Li, W., Koike, K., Academy, Yoshikawa, T., and Nikaido, T. (2005). Rosmarinic acid production of Coleus forskohlii< hairy root cultures. Plant Cell Tiss. Org. Cult. 80, 151–155.

    Article  CAS  Google Scholar 

  10. Kim, H. K., Oh, S. -R., Lee, H. -K., and Huh, H. (2001). Benzothiadiazole enhances the elicitation of rosmarinic acid production in a suspension culture of Agastache rugosa O. Kuntze. Biotechnol. Lett. 23, 55–60.

    Article  Google Scholar 

  11. Karam, N. S., Jawad, F. M., Arikat, N. A., and Shibli, R. A. (2003). Growth and rosmarinic acid accumulation in callus, cell suspension, and root cultures of wild Salvia fruticosa<. Plant Cell Tiss. Org. Cult. 73, 117–121.

    Article  CAS  Google Scholar 

  12. Kirchner, J. G. (1978). Thin Layer Chromatography (2nd Edition), Techniques of Chromatography Series Vol. XIV, Wiley, NY.

    Google Scholar 

  13. Harborne, J. B. (1984). Phytochemical Methods. Chapman and Hall, NY.

    Book  Google Scholar 

  14. Berhow, M. A. (2002). Modern analytical techniques for flavonoid determination, in Flavonoids in the Living Cell (Buslig, B. S. and Manthey, J. A., eds.), Adv. Exp. Med. Biol. Vol. 505, Kluwer/Plenum, NY, pp. 61–76.

    Google Scholar 

  15. Marston, A., and Hostettmann, K. (2006). Separation and quantification of flavonoids, in Flavonoids: Chemistry, Biochemistry and Applications, (Anderson, O. M. and Markham, K. R., eds.), CRC, Boca Raton, FL, pp. 1–36.

    Google Scholar 

  16. Fossen, T., and Anderson, O. M. (2006). Spectroscopic techniques applied to flavonoids, in Flavonoids: Chemistry, Biochemistry and Applications, (Anderson, O. M. and Markham, K. R., eds.), CRC, Boca Raton, FL, pp. 37–142.

    Google Scholar 

Download references

Acknowledgments

The authors would like to thank A. Peterson for plant tissue culture techniques and R. K. Holloway, S. Tisserat and T. Tisserat for chemical analysis. Names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable.

Author information

Authors and Affiliations

  1. Plant Physiologist, New Crops and Processing Technology Research, National Center for Agricultural Utilization Research, Agricultural Research Service-USDA, 1815 N. University St., Peoria, IL, 61604, USA

    Brent Tisserat

Authors
  1. Brent Tisserat
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mark Berhow
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Steven F. Vaughn
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Brent Tisserat .

Editor information

Editors and Affiliations

  1. Department of Applied Biology, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada

    Praveen K. Saxena

Rights and permissions

Reprints and permissions

Copyright information

© 2009 Humana Press, a part of Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Tisserat, B., Berhow, M., Vaughn, S. (2009). Spearmint Plantlet Culture System as a Means to Study Secondary Metabolism. In: Jain, S.M., Saxena, P.K. (eds) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants. Methods in Molecular Biology, vol 547. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-287-2_25

Download citation

  • DOI: https://doi.org/10.1007/978-1-60327-287-2_25

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-286-5

  • Online ISBN: 978-1-60327-287-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation