Summary
Rhodiola rosea is a multipurpose medicinal plant with adaptogenic properties: it increases the body’s nonspecific resistance and normalizes body functions. The commercial interest for roseroot-based products has quickly increased worldwide. Nearly all raw-materials originate from natural populations. As a result of the intensive collection the species has become endangered. Production of the pharmaceutical compounds from the medicinal plants in cell cultures is an alternative to field cultivation. The present protocol describes the establishment of compact callus aggregate culture of Rhodiola rosea and the production of cinnamyl alcohol glycosides through biotransformation.
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References
Ramazanov, Z. and Mar Bernal Suarez, M. (1999). New secrets of effective natural stress and weight management using Rhodiola rosea and Rhododendron caucasicum<. ATN/Safe Goods Publishing, East Canaan, CT, pp. 9–11
Kelly, G. S. (2001). Rhodiola rosea<: a possible plant adaptogen. Alternative Medicine Review 6 (3): 293–302
Brown, R. P., Gerbarg, P. L. and Ramazanov, Z. (2002). Rhodiola rosea<: a phytomedical overview. HerbalGram <56: 40–52
Furmanova, M., Oledzka, H. and Michalska, M. (1995) XXIII Rhodiola rosea< L. (rose-root): in vitro regeneration and the biological activity of roots. In: Bajaj, Y.P.S. (ed.) Biotechnology in Agriculture and Forestry<, Vol. 33, Medicinal and Aromatic Plants VIII. Berlin and Heidelberg, Germany: Springer-Verlag, pp. 412–425
Kurkin, V.A., Zapesochnaya, G.G. and Dubichev, A.G. (1991). Phenylpropanoids of callus culture of Rhodiola rosea<. Khimiya prirodnykh soedinenii 4: 481–490
Furmanova, M., Hartwich, M., Alfermann, A.W., Kozminski, W. and Olejnik, M. (1999). Rosavin as a product of glycosylation by Rhodiola rosea (roseroot) cell cultures. Plant Cell, Tissue Organ and Culture 56: 105–110
Jianfeng, X., Zhiguo, S., and Pusun, F. (1998). Suspension culture of compact callus aggregate of Rhodiola sachalinensis for improved salidroside production. Enzyme and Microbial Technology 23: 20–27
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473–497
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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György, Z., Hohtola, A. (2009). Production of Cinnamyl Glycosides in Compact Callus Aggregate Cultures of Rhodiola rosea Through Biotransformation of Cinnamyl Alcohol. In: Jain, S.M., Saxena, P.K. (eds) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants. Methods in Molecular Biology, vol 547. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-287-2_24
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DOI: https://doi.org/10.1007/978-1-60327-287-2_24
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60327-286-5
Online ISBN: 978-1-60327-287-2
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