Summary
We developed reproducible protocols for micropropagation, callus culture, and root culture of the medicinal plant Phyllanthus urinaria, P. niruri, P. tenellus, P. corcovadensis, P. caroliniensis, P. stipulatus, and P. fraternus by using single node explants. Genotype-linked differences are visible among the Phyllanthus species concerning shoot culture, callus culture, and root culture growth. The protocols developed for phytochemical screening of callus and root extracts of P. urinaria, P. caroliniensis, P. stipulatus, and P. fraternus have shown the production of sterols and triterpenes. Both compounds are known to account for the antinociceptive activity of the methanolic extracts as glochidone and stigmasterol have strong activity against neurogenic and inflammatory pain. Similarly, methanolic callus extracts of P. tenellus, P. niruri and P. corcovadensis have potent analgesic properties, however phenolics are major compounds isolated from these species. The optimized micropropagation, callus culture, and root culture protocols offer the possibility to use cell/root culture techniques for vegetative propagation and secondary metabolite studies.
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Acknowledgments
The authors acknowledges the support of the International Foundation for Science (Sweden, grants D/1265-1, D/1265-2 and D/1265-3 to AMV); The British Council (UK); the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/Brasil); the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (PET/CAPES/Ministry of Education/Brasil); and LG Amaral for classification of plant material.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Catapan, E. et al. (2009). Protocols for In Vitro Culture and Phytochemical Analysis of Phyllanthus Species (Euphorbiaceae). In: Jain, S.M., Saxena, P.K. (eds) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants. Methods in Molecular Biology, vol 547. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-287-2_14
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DOI: https://doi.org/10.1007/978-1-60327-287-2_14
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