Abstract
The first step in the affinity purification of antibodies is usually the immobilization of the corresponding antigen to a solid-phase matrix. Generally, this immobilization is done using some chemically reactive gel material such as cyanogen-bromide activated Sepharose. In fact, for large-scale preparative purifications this is an appropriate method to generate columns with adequate binding capacities and flow properties. However, for many purposes particu-late nitrocellulose (NC) is a promising alternative material, which is characterized by several advantageous properties, some of which are outlined as follows.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Walsh B. J., Sutton R., Wrigley C. W., and Baldo B. A. (1984) Allergen discs prepared from nitrocellulose: detection of IgE binding to soluble and insoluble allergens. J. Immunol. Methods 73, 139–145.
Walsh B. J., Wrigley C. W., Musk A. W., and Baldo B. A. (1985) A comparison of the binding of IgE in the sera of patients with bakers’ asthma to soluble and insoluble wheat-grain proteins. J. Allergy Clin. Immunol. 76, 23–28.
Hammerl P., Hartl A., and Thalhamer J. (1993) Particulate nitrocellulose as a solid phase for protein immobilization in immuno-affinity chromatography. J. Immunol. Methods 165, 59–66.
Levi M. I., Iagovkina V. V., and Bykvareva E. I. (1989) Filament and cellulose acetate columns for affinity chromatography. Lab-Delo. 6, 67–69.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Humana Press Inc.
About this protocol
Cite this protocol
Thalhamer, J., Hammerl, P., Hartl, A. (2000). Nitrocellulose-Based Immunoaffinity Chromatography. In: Bailon, P., Ehrlich, G.K., Fung, WJ., Berthold, W. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 147. Humana Press. https://doi.org/10.1007/978-1-60327-261-2_9
Download citation
DOI: https://doi.org/10.1007/978-1-60327-261-2_9
Publisher Name: Humana Press
Print ISBN: 978-0-89603-694-9
Online ISBN: 978-1-60327-261-2
eBook Packages: Springer Protocols