Abstract
DNA affinity chromatography has been used for the purification of polynucleotides and polynucleotide-binding proteins, including restriction endonucleases, polymerases, proteins involved in recombination, and various transcription factors (reviewed in [1,2]). The earliest supports used were DNA celluloses. P. T. Gilham pioneered the chemical synthesis of homopolymeric DNA-celluloses such as oligo dT cellulose and their use for purifying polynucleotides, especially polyA mRNA, by hybridization (3). Later, Alberts, Litman, and their coworkers adsorbed DNA to cellulose to purify DNA-binding proteins (4,5). Arnt-Jovin and colleagues (6) attached DNA to agarose and Kadonaga and Tijan (7) introduced the addition of competitor DNA to the mobile phase to lessen nonspecific binding. Various other laboratories have attached DNA to Teflon fibers, latex beads, magnetic particles, and other media for DNA affinity chromatography (reviewed in ref. 1).
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References
Jarrett H. W. (1993) Affinity chromatography with nucleic acid polymers. J Chromatogr. 618, 315–339.
Kadonaga J. (1991) Purification of sequence-specific binding proteins by DNA affinity chromatography. Methods Enzymol. 208, 10–23.
Gilham P. (1964) The synthesis of polynucleotide-celluloses and their use in the fractionation of polynucleotides. J. Am. Chem. Soc. 86, 4982–4989.
Alberts B. Amodio F. Jenkins M. Gutman E., and Ferris F. (1968) Studies with DNA-cellulose chromatography. I. DNA-binding proteins from Escherichia coli. Cold Spring Harbor Symp. Quant. Biol. 33, 289–305.
Litman R. (1968) A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lysodeikticus) isolated on deoxyribonucleic acid-cellulose. J. Biol. Chem. 243, 6222–6233.
Arndt-Jovin D. Jovin T. Bahr W. Frischauf A.-M., and Marquardt M. (1975) Covalent attachment of DNA to agarose. Improved synthesis and use in affinity chromatography. Eur. J. Biochem. 54, 411–418.
Kadonaga J. and Tijan R. (1986) Affinity purification of sequence-specific DNA binding proteins. Proc. Natl. Acad. Sci. USA 83, 5889–5893.
Goss T. Bard M., and Jarrett H. (1990) High-performance affinity chromatography of DNA. J. Chromatogr. A 508, 279–287.
Goss T. Bard M., and Jarrett H. (1991) High-performance affinity chromatography of messenger RNA. J. Chromatogr. A 588, 157–164.
Franza B. J. Josephs S. Gilman M. Ryan W., and Clarkson B. (1987) Characterization of cellular proteins recognizing the HIV enhancer using a microscale DNA-affinity precipitation assay. Nature (London) 330, 391–395.
Solomon L. Massom L., and Jarrett H. (1992) Enzymatic syntheses of DNA-silicas using DNA polymerase. Anal. Biochem. 203, 58–69.
DiRusso C. Rogers R. P., and Jarrett H. W. (1994) Novel DNA-Sepharose purification of the FadR transcription factor. J. Chromatogr. A 677, 45–52.
Jarrett H. W. (1995) Preparation of cDNA-silica using reverse transcriptase and its DNA sequence determination. J Chromatogr. A 708, 13–18.
Jarrett H. (1996) Hybrid selection with cDNA-silica. J. Chromatogr. A 742, 87–94.
Sambrook J. Fritsch E., and Maniatis T. (eds.) (1989) Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY.
Massom L. R. and Jarrett H. W. (1992) High-performance affinity chromatography of DNA: II. Porosity effects. J. Chromatogr. A 600, 221–228.
Wallace R. and Miyada C. (1987) Oligonucleotide probes for the screening of recombinant DNA libraries. Methods Enzymol. 152, 432–442.
Jarrett H. W. (1987) Development of N-hydroxysuccinimide ester silica, a novel support for high performance affinity chromatography. J. Chromatogr. A 405, 179–189.
Robinson F. D. Gadgil H., and Jarrett H. W. (1999) Comparative studies on chemically and enzymatically coupled DNA-Sepharose for purification of a lac repressor chimeric fusion protein. J. Chromatogra. A. 849, 403–412.
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Chockalingam, P.S., Jurado, L.A., Darlene, F., Jarrett, H.W. (2000). DNA Affinity Chromatography. In: Bailon, P., Ehrlich, G.K., Fung, WJ., Berthold, W. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 147. Humana Press. https://doi.org/10.1007/978-1-60327-261-2_14
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DOI: https://doi.org/10.1007/978-1-60327-261-2_14
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