Abstract
Polyacrylamide gel electrophoresis (PAGE) is undoubtedly one of the most effective methods for the separation of proteins and is used extensively in the course of protein purification. Usually the separation is carried out in one dimension only and separates according to the size of the protein, i.e., sodium dodecyl sulfate-PAGE (SDS-PAGE) (1). However, to enhance further the separation of complex mixtures, such as whole-cell lysates, two-dimensional PAGE (2D PAGE) will provide an extremely powerful separation of proteins (2).
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© 1996 Humana Press Inc., Totowa, NJ
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Philp, R.J. (1996). Preparation of Peptides for Microsequencing from Proteins in Polyacrylamide Gels. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_68
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DOI: https://doi.org/10.1007/978-1-60327-259-9_68
Publisher Name: Humana Press
Print ISBN: 978-0-89603-338-2
Online ISBN: 978-1-60327-259-9
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