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Fluorescent Protein Staining on Nitrocellulose with Subsequent Immunodetection of Antigen

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Abstract

The transfer of proteins from gels to nitrocellulose or other immobilizing matrices has become increasingly popular as a powerful tool for the subsequent analysis of proteins. Most frequently, the blotted proteins are analyzed for their antigenic properties by Western blotting.

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References

  1. Gershoni, J. M. and Palade, G. E. (1982) Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter. Anal. Biochem. 124, 396–405.

    Article  PubMed  CAS  Google Scholar 

  2. Hancock, K. and Tsang, V. (1983) India ink staining of proteins on nitrocellulose paper. Anal. Biochem. 133, 157–162.

    Article  PubMed  CAS  Google Scholar 

  3. Moeremans, M., Daneels, G. and de Mey, J. (1985) Sensitive colloidal metal (gold or silver) staining of protein blots on nitrocellulose membranes. Anal. Biochem. 145, 315–321.

    Article  PubMed  CAS  Google Scholar 

  4. Salinovich, O. and Montelaro, R. C. (1986) Reversible staining and peptide mapping of proteins transferred to nitrocellulose after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal. Biochem. 156, 341–347.

    Article  PubMed  CAS  Google Scholar 

  5. Burnette, W. N. (1981) “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112, 195–203.

    Article  PubMed  CAS  Google Scholar 

  6. Hawkes, R., Niday, E. and Gordon, J. (1982) A dot immunobindmg assay for monoclonal and other antibodies. Anal. Biochem. 119, 142–147.

    Article  PubMed  CAS  Google Scholar 

  7. Blake, M. S., Johnston, K. H., Russell-Hones, G. J., and Gotschlich, E. C. (1984) A rapid, sensitive method for detection of alkaline phosphatase anti-antibody on Western blots. Anal. Biochem. 136, 175–179.

    Article  PubMed  CAS  Google Scholar 

  8. Brada, D. and Roth, J. (1984) “Golden blot”—detection of polyclonal and monoclonal antibodies bound to antigens on nitrocellulose by protein A-gold complexes. Anal. Biochem. 142, 79–83.

    Article  PubMed  CAS  Google Scholar 

  9. Davis, L. G., Dibner, M. D., and Batley, J. F. (1986) Basic Methods in Molecular Biology. Elsevier, New York.

    Google Scholar 

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© 1996 Humana Press Inc., Totowa, NJ

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Summers, D.F., Szewczyk, B. (1996). Fluorescent Protein Staining on Nitrocellulose with Subsequent Immunodetection of Antigen. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_45

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  • DOI: https://doi.org/10.1007/978-1-60327-259-9_45

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-338-2

  • Online ISBN: 978-1-60327-259-9

  • eBook Packages: Springer Book Archive

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