Abstract
Autoradiography detects the distribution of radioactivity on gels or filters by producing permanent images on photographic film. It is frequently used in a variety of experimental techniques ranging from Southern and Northern blot analysis (1), to visualization of radioactive proteins separated in a sodium dodecyl sulfate (SDS)-Polyacrylamide gels (2), to detection of nuclear factors bound to a labeled DNA probe in gel shift analysis (3), and to localization of DNA bands in sequencing gels (4).
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© 1996 Humana Press Inc., Totowa, NJ
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Circolo, A., Gulati, S. (1996). Autoradiography and Fluorography of Acrylamide Gels. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_36
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DOI: https://doi.org/10.1007/978-1-60327-259-9_36
Publisher Name: Humana Press
Print ISBN: 978-0-89603-338-2
Online ISBN: 978-1-60327-259-9
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