Abstract
In isoelectric focusing (IEF), proteins migrate, under the influence of an electrical field, through a pH gradient created by carrier ampholytes (CA). Separation is based on the characteristic isoelectric point (pI) of each protein, which is the pH at which the protein has zero net charge, and thus zero mobility in the electrical field.
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References
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© 1996 Humana Press Inc., Totowa, NJ
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Pritchett, T.J. (1996). Capillary Isoelectric Focusing with Electro-Osmotic Flow Mobilization. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_24
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DOI: https://doi.org/10.1007/978-1-60327-259-9_24
Publisher Name: Humana Press
Print ISBN: 978-0-89603-338-2
Online ISBN: 978-1-60327-259-9
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