Skip to main content
SpringerLink
Log in
Book cover

The Protein Protocols Handbook pp 127–132Cite as

Two-Dimensional PAGE Using Carrier Ampholyte pH Gradients in the First Dimension

  • Protocol

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Two-dimensional polyacrylamide gel electrophoresis (2D PAGE) is one of the most powerful tools for the separation of complex protein mixtures. This method separates individual proteins and polypeptide chains according to their isoelectric point and molecular weight. This chapter describes the protocol for 2D PAGE using carrier ampholyte pH gradient gel for the isoelectric focusing (IEF) separation (pH gradient 3.5–10.0) and a polyacrylamide gradient gel for the second dimension (1,2).

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Hochstrasser, D. F., Harrington, M. G., Hochstrasser, A. C., Miller, M. J., and Merril, C. R. (1988) Methods for increasing the resolution of two-dimensional protein electrophoresis. Anal. Biochem. 173, 424–435.

    Article  PubMed  CAS  Google Scholar 

  2. Golaz, O. G., Walzer, C., Hochstrasser, D., Bjellqvist, B., Turler, H., and Balant, L. (1992) Red blood cell protein map: a comparison between carrier-ampholyte pH gradient and immobilized pH gradient, and identification of four red blood cell enzymes. Appl. Theor. Electrophoresis 3, 77–82.

    CAS  Google Scholar 

  3. Oakley, B. R., Kirsch, D. R., and Morris, N. R. (1990) A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal. Biochem. 105, 361–363.

    Article  Google Scholar 

  4. Hochstrasser, D. F. and Merril, C. R. (1988) Catalysts for polyacrylamide gel polymerization and detection of proteins by silver staining. Appl. Theor. Electrophoresis 1, 35–40.

    CAS  Google Scholar 

  5. Rabilloud, T. (1992) A comparison between low background silver diammine and silver nitrate protein stains. Electrophoresis 13, 429–439.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Clinical Research Unit, Psychiatric Institutions of Geneva, Switzerland

    Patricia Gravel

  2. Central Clinical Chemistry Laboratory, University Hospital of Geneva, Switzerland

    Olivier Golaz

Authors
  1. Patricia Gravel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Olivier Golaz
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    John M. Walker

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Gravel, P., Golaz, O. (1996). Two-Dimensional PAGE Using Carrier Ampholyte pH Gradients in the First Dimension. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_20

Download citation

  • DOI: https://doi.org/10.1007/978-1-60327-259-9_20

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-338-2

  • Online ISBN: 978-1-60327-259-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation