Abstract
The most accurate method of determining protein concentration is probably acid hydrolysis followed by amino acid analysis. Most other methods are sensitive to the amino acid composition of the protein, and absolute concentrations cannot be obtained. The procedure of Lowry et al. (1) is no exception, but its sensitivity is moderately constant from protein to protein, and it has been so widely used that Lowry protein estimations are a completely acceptable alternative to a rigorous absolute determination in almost all circumstances where protein mixtures or crude extracts are involved.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Lowry, O. H., Rosebrough, N. J., Fair, A. L., and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275.
Waterborg, J. H. and Matthews, H. R. (1984) The Burton Assay for DNA, in Methods in Molecular Biology, vol. 2: Nucleic Acids (Walker, J. M., ed.), Humana, Totowa, NJ, pp. 1–3.
Peterson, G. L. (1983) Determination of total protein. Methods Enzymol. 91, 95–121.
Markwell, M. A. K., Haas, S. M., Tolbert, N. E., and Bieber, L. L. (1981) Protein determination in membrane and lipoprotein samples. Methods Enzymol. 72, 296–303.
Hess, H. H., Lees, M. B., and Derr, J. E. (1978) A linear Lowry-Folin assay for both water-soluble and sodium dodecyl sulfate-solubilized proteins. Anal. Biochem. 85, 295–300.
Larson, E., Howlett, B., and Jagendorf, A. (1986) Artificial reductant enhancement of the Lowry method for protein determination. Anal. Biochem. 155, 243–248.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Waterborg, J.H., Matthews, H.R. (1996). The Lowry Method for Protein Quantitation. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_2
Download citation
DOI: https://doi.org/10.1007/978-1-60327-259-9_2
Publisher Name: Humana Press
Print ISBN: 978-0-89603-338-2
Online ISBN: 978-1-60327-259-9
eBook Packages: Springer Book Archive