Abstract
Steady-state measurements of fluorescence polarization have been widely adopted in the field of high-throughput screening for the study of biomolecular interactions. This chapter reviews the basic theory of fluorescence polarization, the underlying principle for using fluorescence polarization to study interactions between small-molecule fluorophores and macromolecular targets, and representative applications of fluorescence polarization in high-throughput screening.
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Acknowledgments
The authors would like to thank Ray Unwalla of Wyeth Research for molecular modeling of E-76 and E-76 based probes; Rebecca Shirk and Belew Mekonnen of Wyeth Research for collaboration on the TF/FVIIa; Shannon Stahler, Nina Kadakia, Gary Kalgaonkar, William Martin, Mariya Gazumyan, Pedro Sobers, and Jim LaRocque of Wyeth Research for contribution to the NR project; and Richard Harrison of Wyeth Research for critical review of the chapter.
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Huang, X., Aulabaugh, A. (2009). Application of Fluorescence Polarization in HTS Assays. In: Janzen, W., Bernasconi, P. (eds) High Throughput Screening. Methods in Molecular Biology, vol 565. Humana Press. https://doi.org/10.1007/978-1-60327-258-2_6
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DOI: https://doi.org/10.1007/978-1-60327-258-2_6
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