Abstract
Cystic fibrosis (CF) is the most common autosomal recessive disease in the USA and Europe, whose life-limiting phenotype is manifest on epithelial cells throughout the body. The principal cause of morbidity and mortality is a massively proinflammatory condition in the lung. The mutation responsible for most cases of CF is [ΔF508]CFTR. However, the penetrance of the disease is quite variable, and adverse events leading to hospitalization cannot be easily predicted. Thus, there is a strong need for prognostic endpoints that might serve to identify impending clinical problems long before they happen. Our approach has been to search for proteomic signatures in easily accessed biological fluids that might identify the molecular basis for adverse events. We describe here a workflow that begins with patient-derived bronchial brush biopsies and progresses to analysis of serum and plasma from patients on antibody microarrays.
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References
Welsh, M. J., Ramsey, B. W., Accurso, F., Cutting, G. R. (2001) Cystic Fibrosis, in The Metabolic Basis of Inherited Disease (Scriver, C. L., Beaudet, A. L., Valle, D., and Sly, W. S., ed.), McGraw-Hill, New York, pp. 5121–5188.
Cystic Fibrosis Foundation (2008) Patient Registry 2008 Annual Report. Bethesda, MD.
Liou, T. G., Adler, F. R., Fitzsimmons, S. C., Cahill, B. C., Hibbs, J. R., Marshall, B. C. (2001) Predictive 5-year survivorship model of cystic fibrosis. Am J Epidemiol 153, 345–52.
Espina, V., Wulfkuhle, J. D., Calvert, V. S., VanMeter, A., Zhou, W., Coukos, G. et al. (2006) Laser-capture microdissection. Nat Protoc 1, 586–603.
Emmert-Buck, M. R., Bonner, R. F., Smith, P. D., Chuaqui, R. F., Zhuang, Z., Goldstein, S. R. et al. (1996) Laser capture microdissection. Science 274, 998–1001.
Orba, Y., Tanaka, S., Nishihara, H., Kawamura, N., Itoh, T., Shimizu, M. et al. (2003) Application of laser capture microdissection to cytologic specimens for the detection of immunoglobulin heavy chain gene rearrangement in patients with malignant lymphoma. Cancer 99, 198–204.
Pollard, H. B., Srivastava, M., Eidelman, O., Jozwik, C., Rothwell, S. W., Mueller, G. P. et al. (2007) Protein microarray platforms for clinical proteomics. Proteomics Clin Appl 1, 934–52.
Srivastava, M., Eidelman, O., Jozwik, C., Paweletz, C., Huang, W., Zeitlin, P. L. et al. (2006) Serum proteomic signature for cystic fibrosis using an antibody microarray platform. Mol Genet Metab 87, 303–10.
Eidelman, O., Jozwik, C., Huang, W., Srivastava, M., Rothwell, S. W., Jacobowitz, D. M. et al. (2010) Gender dependence for a subset of the low-abundance signaling proteome in human platelets. Hum Genomics Proteomics 2010, 164906.
Miller, J. C., Zhou, H., Kwekel, J., Cavallo, R., Burke, J., Butler, E. B. et al. (2003) Antibody microarray profiling of human prostate cancer sera: antibody screening and identification of potential biomarkers. Proteomics 3, 56–63.
Hamelinck, D., Zhou, H., Li, L., Verweij, C., Dillon, D., Feng, Z. et al. (2005) Optimized normalization for antibody microarrays and application to serum-protein profiling. Mol Cell Proteomics 4, 773–84.
Espina, V., Wulfkuhle, J., Liotta, L. A. (2009) Application of laser microdissection and reverse-phase protein microarrays to the molecular profiling of cancer signal pathway networks in the tissue microenvironment. Clin Lab Med 29, 1–13.
Iyer, E. P., Cox, D. N. (2010) Laser capture micro-dissection of Drosophila peripheral neurons. J Vis Exp 24(39). pii: 2016. doi: 10.3791/2016.
Neblock, D. S., Chang, C. H., Mascelli, M. A., Fleek, M., Stumpo, L., Cullen, M. M. et al. (1992) Conjugation and evaluation of 7E3 x P4B6, a chemically cross-linked bispecific F(ab’)2 antibody which inhibits platelet aggregation and localizes tissue plasminogen activator to the platelet surface. Bioconjug Chem 3, 126–31.
Haab, B. B. (2001) Advances in protein microarray technology for protein expression and interaction profiling. Curr Opin Drug Discov Devel 4, 116–23.
Yu, X., Schneiderhan-Marra, N., Joos, T. O. (2010) Protein microarrays for personalized medicine. Clin Chem 56, 376–87.
Yu, X., Schneiderhan-Marra, N., Hsu, H. Y., Bachmann, J., Joos, T. O. (2009) Protein microarrays: effective tools for the study of inflammatory diseases. Methods Mol Biol 577, 199–214.
Qin, S., Qiu, W., Ehrlich, J. R., Ferdinand, A. S., Richie, J. P., O’Leary M, P. et al. (2006) Development of a “reverse capture” autoantibody microarray for studies of antigen-autoantibody profiling. Proteomics 6, 3199–209.
Ehrlich, J. R., Qin, S., Liu, B. C. (2006) The ‘reverse capture’ autoantibody microarray: a native antigen-based platform for autoantibody profiling. Nat Protoc 1, 452–60.
Rai, A. J., Gelfand, C. A., Haywood, B. C., Warunek, D. J., Yi, J., Schuchard, M. D. et al. (2005) HUPO Plasma Proteome Project specimen collection and handling: towards the standardization of parameters for plasma proteome samples. Proteomics 5, 3262–77.
Tusher, V. G., Tibshirani, R., Chu, G. (2001) Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci USA 98, 5116–21.
Hartmann, M., Roeraade, J., Stoll, D., Templin, M. F., Joos, T. O. (2009) Protein microarrays for diagnostic assays. Anal Bioanal Chem 393, 1407–16.
Ellington, A. A., Kullo, I. J., Bailey, K. R., Klee, G. G. (2009) Measurement and quality control issues in multiplex protein assays: a case study. Clin Chem 55, 1092–9.
Ellington, A. A., Kullo, I. J., Bailey, K. R., Klee, G. G. (2010) Antibody-based protein multiplex platforms: technical and operational challenges. Clin Chem 56, 186–93.
Kricka, L. J., Master, S. R. (2008) Validation and quality control of protein microarray-based analytical methods. Mol Biotechnol 38, 19–31.
Kricka, L. J., Master, S. R. (2009) Quality control and protein microarrays. Clin Chem 55, 1053–5.
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Jozwik, C.E. et al. (2012). Antibody Microarrays: Analysis of Cystic Fibrosis. In: Espina, V., Liotta, L. (eds) Molecular Profiling. Methods in Molecular Biology, vol 823. Humana Press. https://doi.org/10.1007/978-1-60327-216-2_12
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DOI: https://doi.org/10.1007/978-1-60327-216-2_12
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