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2D PAGE: Sample Preparation and Fractionation pp 113–130Cite as

Sample Preparation of Culture Medium from Madin-Darby Canine Kidney Cells

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 425))

Summary

A reproducible, standardized and simple sample preparation methodology is the key to successful two-dimensional gel electrophoresis (2-DE). This chapter describes step-by-step the sample preparation of culture medium from Madin-Darby canine kidney (MDCK) cells. Tips and tricks are given to circumvent possible pitfalls.

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Author information

Authors and Affiliations

  1. University of Berne, Berne, Switzerland

    Daniel Ambort, Daniel Lottaz & Erwin Sterchi

Authors
  1. Daniel Ambort
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  2. Daniel Lottaz
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  3. Erwin Sterchi
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Editor information

Editors and Affiliations

  1. Bio-Rad Laboratories GmbH, Munich, Germany

    Anton Posch

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Ambort, D., Lottaz, D., Sterchi, E. (2008). Sample Preparation of Culture Medium from Madin-Darby Canine Kidney Cells. In: Posch, A. (eds) 2D PAGE: Sample Preparation and Fractionation. Methods in Molecular Biology™, vol 425. Humana Press. https://doi.org/10.1007/978-1-60327-210-0_10

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  • DOI: https://doi.org/10.1007/978-1-60327-210-0_10

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-209-4

  • Online ISBN: 978-1-60327-210-0

  • eBook Packages: Springer Protocols

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