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Characterization of Nanoparticles by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

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Characterization of Nanoparticles Intended for Drug Delivery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 697))

Abstract

Determining the molecular weight of nanoparticles can be challenging. The molecular weight characterization of dendrimers, for example, with varying covalent and noncovalent modifications is critical to their use as therapeutics. As such, we describe in this chapter a protocol for the analysis of these molecules by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).

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Acknowledgments

This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under contract N01-CO-12400. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.

Jennifer Hall, Ph.D., Nanotechnology Characterization Laboratory, ATP, SAIC-Frederick, NCI-Frederick, provided useful comments and critical reading of the manuscript.

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Authors and Affiliations

  1. Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC – Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD, USA

    Uma Ramalinga, Jeffrey D. Clogston & Anil K. Patri

  2. Protein Chemistry Laboratory, Advanced Technology Program, SAIC – Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD, USA

    John T. Simpson

Authors
  1. Uma Ramalinga
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  2. Jeffrey D. Clogston
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  3. Anil K. Patri
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  4. John T. Simpson
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Corresponding author

Correspondence to John T. Simpson .

Editor information

Editors and Affiliations

  1. SAIC-Frederick, Inc., Nanotechnology Characterization Lab, National Cancer Institute at Frederick, Boyles Street 1050, Frederick, 21702, Maryland, USA

    Scott E. McNeil

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Ramalinga, U., Clogston, J.D., Patri, A.K., Simpson, J.T. (2011). Characterization of Nanoparticles by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry. In: McNeil, S. (eds) Characterization of Nanoparticles Intended for Drug Delivery. Methods in Molecular Biology, vol 697. Humana Press. https://doi.org/10.1007/978-1-60327-198-1_5

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  • DOI: https://doi.org/10.1007/978-1-60327-198-1_5

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-197-4

  • Online ISBN: 978-1-60327-198-1

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