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Characterization of Nanoparticles Intended for Drug Delivery pp 167–172Cite as

Monitoring Nanoparticle-Treated Hepatocarcinoma Cells for Apoptosis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 697))

Abstract

This chapter describes a method for monitoring nanoparticle treated human hepatocarcinoma cells (Hep G2) for apoptosis. The protocol utilizes a fluorescent method to determine the degree of caspase-3 activation.

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References

  1. Thompson, C.B. (1995) Apoptosis in the pathogenesis and treatment of disease. Science 267, 1456–1462.

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  2. Jacobson, M.D., Weil, M. and Raff, M.C. (1997) Programmed cell death in animal development. Cell 88, 347–354.

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  3. Kuida, K., Zheng, T.S., Kuan C.Y., Yang, D., Karasuyama, H., Rakic, P. and Flavell, R.A. (1996) Decreased apoptosis in the brain and premature lethality in CPP32-deficient mice. Nature 384, 368–372.

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Acknowledgments

This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under contract N01-CO-12400. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.

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Authors and Affiliations

  1. Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC – Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD, USA

    Timothy M. Potter & Stephan T. Stern

Authors
  1. Timothy M. Potter
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  2. Stephan T. Stern
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Corresponding author

Correspondence to Stephan T. Stern .

Editor information

Editors and Affiliations

  1. SAIC-Frederick, Inc., Nanotechnology Characterization Lab, National Cancer Institute at Frederick, Boyles Street 1050, Frederick, 21702, Maryland, USA

    Scott E. McNeil

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© 2011 Springer Science+Business Media, LLC

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Potter, T.M., Stern, S.T. (2011). Monitoring Nanoparticle-Treated Hepatocarcinoma Cells for Apoptosis. In: McNeil, S. (eds) Characterization of Nanoparticles Intended for Drug Delivery. Methods in Molecular Biology, vol 697. Humana Press. https://doi.org/10.1007/978-1-60327-198-1_17

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  • DOI: https://doi.org/10.1007/978-1-60327-198-1_17

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-197-4

  • Online ISBN: 978-1-60327-198-1

  • eBook Packages: Springer Protocols

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