Abstract
This chapter provides a protocol for quantitative analysis of gold in solution as well as gold uptake by macrophages. A 96-well fluorescence assay was developed to be able to determine gold concentrations for a given gold nanoparticle as well as quantify the degree of gold nanoparticle uptake by macrophages. This assay detects a decrease in the fluorescence of a dye upon forming a complex with gold and a ligand. The decrease in fluorescence is proportional to the amount of gold. This protocol provides a preliminary and qualitative first-step alternative for the determination of gold nanoparticle concentration without requiring expensive, time-consuming methods such as inductively coupled plasma mass spectroscopy. This protocol can be used to support the uptake studies by the light microscopy method described in Chapter 23. This assay requires 200 μL of each test nanoparticle at a concentration of the users choosing. Up to 32 variations of test nanoparticles can be evaluated in duplicate in one assay run.
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References
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Acknowledgments
This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under contract N01-CO-12400. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government.
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Aggarwal, P., Dobrovolskaia, M.A. (2011). Gold Nanoparticle Quantitation via Fluorescence in Solution and Cell Culture. In: McNeil, S. (eds) Characterization of Nanoparticles Intended for Drug Delivery. Methods in Molecular Biology, vol 697. Humana Press. https://doi.org/10.1007/978-1-60327-198-1_14
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DOI: https://doi.org/10.1007/978-1-60327-198-1_14
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Publisher Name: Humana Press
Print ISBN: 978-1-60327-197-4
Online ISBN: 978-1-60327-198-1
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