Abstract
This chapter describes a procedure for quantitative determination of microbial contamination of a nanoparticle formulation. The protocol includes tests for yeast, mold, and bacteria using Millipore sampler devices. This approach is primarily intended to avoid contamination of cell cultures and transmitting potential microbial contaminants to animals in preclinical studies of efficacy, biodistribution, and toxicity. Other methods common to microbiology will likely work equally well.
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References
Samplers, dilution kits and swab test kits user guide. P15325, Rev. D., 8/99 Millipore Corp.
USP Standard 51 (2007) Antimicrobial effectiveness testing USP 30 NF 25 1.
USP Standard 61 (2007) Microbial limit tests USP 30 NF 25 1.
USP Standard 71 (2007) Sterility tests USP 30 NF 25 1.
Acknowledgments
This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under contract N01-CO-12400. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.
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Potter, T.M., Dobrovolskaia, M.A. (2011). Analysis of Microbial Contamination in Nanoparticle Formulations. In: McNeil, S. (eds) Characterization of Nanoparticles Intended for Drug Delivery. Methods in Molecular Biology, vol 697. Humana Press. https://doi.org/10.1007/978-1-60327-198-1_13
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DOI: https://doi.org/10.1007/978-1-60327-198-1_13
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Publisher Name: Humana Press
Print ISBN: 978-1-60327-197-4
Online ISBN: 978-1-60327-198-1
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