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Animal Models of Acute Neurological Injuries pp 187–196Cite as

Middle Cerebral Artery Occlusion Suture Model for Mice

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This chapter introduces the procedure, assessment, application, limitations, and technical considerations of the popular focal brain ischemia model in mice, the middle cerebral artery occlusion (MCAO) suture model. Preparation of the suture, surgical procedures, examination of neurological function, and infarct volume assessment are described in detail.

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References

  1. Longa EZ, Weinstein PR, Carlson S, Cummins R. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke 1989; 20:84–91

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  2. Yang G, Chan PH, Chen J, et al. Human copper-zinc superoxide dismutase trans-genic mice are highly resistant to reperfusion injury after focal cerebral ischemia. Stroke 1994; 25:165–70

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  3. Connolly ES Jr., Winfree CJ, Stern DM, Solomon RA, Pinsky DJ. Procedural and strain-related variables significantly affect outcome in a murine model of focal cerebral ischemia. Neurosurgery 1996; 38:523–31; discussion 532

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  4. Tureyen K, Vemuganti R, Sailor KA, Dempsey RJ. Ideal suture diameter is critical for consistent middle cerebral artery occlusion in mice. Neurosurgery 2005; 56:196–200; discussion 196–200

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  5. Maeda K, Mies G, Olah L, Hossmann KA. Quantitative measurement of local cerebral blood flow in the anesthetized mouse using intraperitoneal [14C]iodoantipyrine injection and final arterial heart blood sampling. J Cereb Blood Flow Metab 2000; 20:10–4

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Author information

Authors and Affiliations

  1. Department of Neurology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Feng Zhang, Steven H. Graham & Jun Chen

Authors
  1. Feng Zhang
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  2. Steven H. Graham
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  3. Jun Chen
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Editor information

Editors and Affiliations

  1. Department of Neurology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Jun Chen M.D.

  2. Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, USA

    Zao C. Xu M.D., Ph.D.

  3. Spinal Cord and Brain Injury Research Group Stark Neurosciences Research Institute Department of Neurological Surgery, Indiana University School of Medicine, Indianapolis, IN, USA

    Xiao-Ming Xu M.D., Ph.D.

  4. Department of Physiology and Pharmacology Department of Neurosurgery Department of Anesthesiology, Loma Linda University Medical School, Loma Linda, CA, USA

    John H. Zhang M.D., Ph.D.

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© 2009 Humana Press, a part of Springer Science + Business Media, LLC

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Cite this protocol

Zhang, F., Graham, S.H., Chen, J. (2009). Middle Cerebral Artery Occlusion Suture Model for Mice. In: Chen, J., Xu, Z.C., Xu, XM., Zhang, J.H. (eds) Animal Models of Acute Neurological Injuries. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-185-1_15

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  • DOI: https://doi.org/10.1007/978-1-60327-185-1_15

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-184-4

  • Online ISBN: 978-1-60327-185-1

  • eBook Packages: Springer Protocols

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