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Inositol Phosphates and Lipids pp 21–46Cite as

HPLC Separation of Inositol Polyphosphates

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 645))

Abstract

High performance liquid chromatography (HPLC) is an essential analytical tool in the study of the large number of inositol phosphate isomers. This chapter focuses on the separation of inositol polyphosphates from [3H]myo-inositol labeled tissues and cells. We review the different HPLC columns that have been used to separate inositol phosphates and their advantages and disadvantages. We describe important elements of sample preparation for effective separations and give examples of how changing factors, such as pH, can considerably improve the resolving ability of the HPLC chromatogram.

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Acknowledgments

The authors would like to thank Prof. R.H. Michell and Dr. C.J. Kirk for permission to use data obtained while C.J.B was a research fellow in their laboratory. The authors own work was supported by grants from Karolinska Institutet, Novo Nordisk Foundation, the Swedish Research Council, the Swedish Diabetes Association, EFSD, The Family Erling-Persson Foundation, Berth von Kantzow’s Foundation, and EuroDia (LSHM-CT-2006-518153).

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Authors and Affiliations

  1. The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Stockholm, Sweden

    Christopher J. Barker, Christopher Illies & Per-Olof Berggren

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  1. Christopher J. Barker
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  2. Christopher Illies
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  3. Per-Olof Berggren
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Editors and Affiliations

  1. Dept. Molecular Medicine & Surgery, Rolf Luft Research Ctr. for Diabetes, Karolinska University Hospital Solna, Stockholm, Sweden

    Christopher J. Barker

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Barker, C.J., Illies, C., Berggren, PO. (2010). HPLC Separation of Inositol Polyphosphates. In: Barker, C. (eds) Inositol Phosphates and Lipids. Methods in Molecular Biology, vol 645. Humana Press. https://doi.org/10.1007/978-1-60327-175-2_2

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  • DOI: https://doi.org/10.1007/978-1-60327-175-2_2

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-174-5

  • Online ISBN: 978-1-60327-175-2

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