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Mesenchymal Stem Cells from Adult Bone Marrow

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Mesenchymal Stem Cells

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 449))

Abstract

Mesenchymal stem cells (MSCs), sometimes referred to as marrow stromal cells or multipotential stromal cells, represent a class of adult progenitor cells capable of differentiation to several mesenchymal lineages. They can be isolated from many tissues although bone marrow has been used most often. The MSCs may prove useful for repair and regeneration of a variety of mesenchymal tissues such as bone, cartilage, muscle, marrow stroma, and the cells produce useful growth factors and cytokines that may help repair additional tissues. There is also evidence for their differentiation to nonmesenchymal lineages, but that work will not be considered here. This chapter will provide the researcher with some background, and then provide details on MSC isolation, expansion and multilineage differentiation. These are the beginning steps toward formulating tissue repair strategies. The methods provided here have been used in many laboratories around the world and the reader can begin by following the methods presented here, and then test other methods if these prove unsatisfactory for your intended purpose.

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Authors and Affiliations

  1. Cardiology Dept of Medicine, Johns Hopkins School of Medicine, Baltimore, MD, USA

    Mark F. Pittenger

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  1. Mark F. Pittenger
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Editors and Affiliations

  1. Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA

    Darwin J. Prockop , Bruce A. Bunnell  & Donald G. Phinney ,  & 

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Pittenger, M.F. (2008). Mesenchymal Stem Cells from Adult Bone Marrow. In: Prockop, D.J., Bunnell, B.A., Phinney, D.G. (eds) Mesenchymal Stem Cells. Methods in Molecular Biology™, vol 449. Humana Press. https://doi.org/10.1007/978-1-60327-169-1_2

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  • DOI: https://doi.org/10.1007/978-1-60327-169-1_2

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-771-6

  • Online ISBN: 978-1-60327-169-1

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