Skip to main content
SpringerLink
Log in
Book cover

Bacteriophages pp 77–80Cite as

Enumeration of Bacteriophages by the Direct Plating Plaque Assay

  • Protocol

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 501))

Abstract

A method is described for determination of the concentration of infectious phage particles by the direct plating plaque assay, which is simpler and faster than the double agar overlay plaque procedure outlined in the previous chapter.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   239.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

Reference

  1. d’Herelle, F. 1917. Sur un microbe invisible antagoniste des bacilles dysent’eriques. Comptes rendus Acad’emie Sciences 165: 373–375.

    Google Scholar 

  2. Adams, M.D. 1959. Bacteriophages. Interscience Publishers, Inc., New York.

    Google Scholar 

  3. Arber, W.L., L. Enquist, B. Hohn, N.E. Murray, and K. Murray. 1983. Experimental Methods for use with Lambda., p. 433. In Lambda II. Cold Spring Harbor Laboratory Press, Cold Spring Harbor Laboratory, NY.

    Google Scholar 

  4. Sambrook, J. and D.W. Russell. 2001. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Press, Cold Spring Harbor, New York.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Public Health Agency of Canada, Laboratory for Foodborne Diseases, Guelph, Ontario, Canada

    Amanda Mazzocco, Erika Lingohr & Roger P. Johnson

  2. Pro-Lab Diagnostics, Richmond Hill, Ontario, Canada

    Thomas E. Waddell

Authors
  1. Amanda Mazzocco
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thomas E. Waddell
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Erika Lingohr
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Roger P. Johnson
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Leicester, Leicester, UK

    Martha R.J. Clokie

  2. Public Health Agency of Canada, Guelph, Ontario, Canada

    Andrew M. Kropinski

Rights and permissions

Reprints and permissions

Copyright information

© 2009 Humana Press, a part of Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Mazzocco, A., Waddell, T.E., Lingohr, E., Johnson, R.P. (2009). Enumeration of Bacteriophages by the Direct Plating Plaque Assay. In: Clokie, M.R., Kropinski, A.M. (eds) Bacteriophages. Methods in Molecular Biology™, vol 501. Humana Press. https://doi.org/10.1007/978-1-60327-164-6_8

Download citation

  • DOI: https://doi.org/10.1007/978-1-60327-164-6_8

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-682-5

  • Online ISBN: 978-1-60327-164-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation