Abstract
A general procedure is described for the analysis of gene expression of Candida albicans cultured in a mouse infection model. This technique involves first infecting mice with Candida and subsequently harvesting blood and other tissue at specific time points during infection. The tissues are homogenized and the infecting Candida isolated. Finally, RNA is extracted from recovered Candida cells and subjected to microarray analysis.
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Kruppa, M.D. (2009). Candida albicans Gene Expression in an In Vivo Infection Model. In: Cihlar, R.L., Calderone, R.A. (eds) Candida albicans. Methods in Molecular Biology, vol 499. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-151-6_9
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DOI: https://doi.org/10.1007/978-1-60327-151-6_9
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-58829-760-0
Online ISBN: 978-1-60327-151-6
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