Abstract
Genetic transformation is the primary method of genetic manipulation of Candida albicans. The lack of a complete sexual cycle prevents application of classical genetic analyses. However, transformation permits introduction into the genome of a wide variety of defined mutations including deletions, insertions, and fusions. Although several methods of transformation are available, the lithium-cation-induced transformation method described here is the most commonly used.
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Ramon, A.M., Fonzi, W.A. (2009). Genetic Transformation of Candida albicans . In: Cihlar, R.L., Calderone, R.A. (eds) Candida albicans. Methods in Molecular Biology, vol 499. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-151-6_16
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DOI: https://doi.org/10.1007/978-1-60327-151-6_16
Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-60327-151-6
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