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Eicosanoids: Generation and Detection in Mammalian Cells

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Lipid Signaling Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 462))

Abstract

Eicosanoids are 20-carbon lipids generated by the oxidation of arachidonic acid that are involved in physiological signaling in virtually all organ systems. Three primary enzymatic pathways are responsible for their synthesis in mammalian cells: lipoxygenase, cyclooxygenase, and cytochrome P450. They signal through receptor-dependent pathways, and their dysregulation is central to numerous pathological states including cancer and inflammation. Recent advances in their detection and analysis using mass spectrometry have made the study of these molecules more accessible to the research community in general. This review focuses on the available methods for the detection and analysis of eicosanoids and aims to act as a guide for those wishing to approach the analysis of eicosanoids for their own research.

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Author information

Authors and Affiliations

  1. Department of Medical Biochemistry and Immunology, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK

    Valerie B. O’Donnell

Authors
  1. Valerie B. O’Donnell
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  2. Ben Maskrey
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  3. Graham W. Taylor
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Corresponding author

Correspondence to Valerie B. O’Donnell .

Editor information

Editors and Affiliations

  1. Dept. Cell Biophysics, Cancer Research UK, Lincoln's Inn Fields 44, London, WC2A 3PX, United Kingdom

    Banafshé Larijani

  2. Fac. Natural Sciences, Div. Cell & Molecular Biology, Imperial College London, London, SW7 2AZ, United Kingdom

    Rudiger Woscholski

  3. Rye St. Antony School, Pullens Lane, Oxford, OX3 0BY, United Kingdom

    Colin A. Rosser

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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O’Donnell, V.B., Maskrey, B., Taylor, G.W. (2009). Eicosanoids: Generation and Detection in Mammalian Cells. In: Larijani, B., Woscholski, R., Rosser, C. (eds) Lipid Signaling Protocols. Methods in Molecular Biology, vol 462. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-115-8_1

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  • DOI: https://doi.org/10.1007/978-1-60327-115-8_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-58829-727-3

  • Online ISBN: 978-1-60327-115-8

  • eBook Packages: Springer Protocols

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