Abstract
A protocol for micropropagation of begonia was established utilizing a thin cell layer (TCL) system. This system has been employed to produce several thousand shoots per sample. Explant size and position, and plant growth regulators (PGRs) contribute to the tissue morphogenesis. By optimizing the size of the tissue and applying an improved selection procedure, shoots were elongated in 8 weeks of culture, with an average number of 210 ± 9.7 shoots per segment. This system has facilitated a number of studies using TCL as a model for micropropagation and will enable the large-scale production of begonia. On an average, the best treatment would allow production of about 10,000 plantlets by the micropropagation of the axillary buds of one plant with five petioles, within a period of 8 months.
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References
Inoue Y (1983) Encyclopedia of horticulture, vol 2B. Seibundo-Shinkosha, Tokyo
Willis JC (1973) A dictionary of the flowering plants and ferns, 8th edn. Cambridge University Press, Cambridge, England Revised by HK Airy Sahw
Heide OM (1964) Effects of light and temperature on the regeneration ability of Begonia leaf cuttings. Plant Physiol 17:789–804
Maier U, Sattler R (1977) The structure of the epiphyllous appendages Begonia hispida var. cucullifera. Can J Bot 55:264–280
Chlyah A, Van Tran Thanh K (1968) Budding capacity of undetached Begonia rex leaves. Nature 218:493
Van Tran Thanh M (1973) In vitro control of de novo flower, bud, root and callus differentiation from excised epidermal tissues. Nature 246:44–45
Nhut DT, Aswath CR, da Silva JAT, Le VL, Thorpe T, Tran Thanh Van K (2003) Tobacco thin cell layer morphogenesis. In: Nhut DT, Le BV, Tran Thanh Van K, Thorpe T (eds) Thin cell layer culture system: regeneration and transformation applications. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 285–342
Tran Thanh Van K, Lie-Schricke H, Marcotte JL, Trinh TH (1986) Winged bean (Psophocarpus tetragonolobus L. DC. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. Crop I, vol 2. Springer, Berlin Heidelberg, New York, USA, pp 556–567
Juillien F, Wyndaele R (1992) Precocious in vitro flowering of soybean cotyledonary node. J Plant Physiol 140:251–253
Das AB, Rout GR, Das P (1995) In vitro somatic embryogenesis from callus culture of the timber yielding tree Hardwickia binata Roxb. Plant Cell Rep 15:147–149
Carimi F, de Pasquale F, Crescimanno FG (1999) Somatic embryogenesis and plant regeneration from pistil thin cell layers of Citrus. Plant Cell Rep 18:935–940
Juillien F, Van Tran Thanh K (1994) Micropropagation and embryoid formation from young leaves of Bambusa glaucescens ‘Golden Goddess’. Plant Sci 98:199–207
Sugimura Y, Salvana MJ (1989) Induction and growth of callus derived from rachilla explants of young inflorescences of coconut palm. Can J Bot 67:272–274
Nhut DT, Bui VL, Van Tran Thanh K (2000) Somatic embryogenesis and direct shoot regeneration of rice (Oryza sativa L.) using thin cell layer culture of apical meristematic tissue. J Plant Physiol 157:559–565
Bui VL, Hang Phuong NT, Anh Hong LT, Van Tran Thanh K (1999) High frequency shoot regeneration from Rhynchostylis gigantea (Orchidaceae) using thin cell layers. Plant Growth Regul 28:179–185
Chlyah A, Van Tran Thanh K (1975) Different reactivity in epidermal cells of Begonia rex excised and grown in vitro. Plant Physiol 35:16–20
Mikkelsen EP, Sink KC (1978) Histology of adventitious shoot and root formation on leaf-petiole cuttings of Begonia × hiemalis Fotsch Aphrodite Peach. Sci Hortic 8:179–192
Takayama S, Misawa M (1981) Mass propagation of Begonia × hiemalis plantlets by shake culture. Plant Cell Physiol 22:461–467
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479
Nhut DT, Hai NT, Huong DTQ, Hang NTT, da Silva JAT (2005) Thidiazuron induces high frequency shoot regeneration in begonia petiole-leaf transverse thin cell layer culture. Prop Ornament Plants 5:149–155
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Nhut, D.T., Hai, N.T., Phan, M.X. (2010). A Highly Efficient Protocol for Micropropagation of Begonia tuberous . In: Jain, S., Ochatt, S. (eds) Protocols for In Vitro Propagation of Ornamental Plants. Methods in Molecular Biology, vol 589. Humana Press. https://doi.org/10.1007/978-1-60327-114-1_2
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DOI: https://doi.org/10.1007/978-1-60327-114-1_2
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