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Protocols for In Vitro Propagation of Ornamental Plants pp 153–162Cite as

In Vitro Regeneration and Multiplication of Passiflora Hybrid “Guglielmo Betto”

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 589))

Abstract

With more than 450 species, Passiflora is the most important genus of the family Passifloraceae. It comprises many species grown for their edible fruits, for their high ornamental value, and further for the therapeutic properties. With their striking exotic flowers, they are of particular interest for the floriculture market. With the aim of evaluating the in vitro propagation of an Italian ornamental hybrid, axillary tendrils of Passiflora “Guglielmo Betto” M. Vecchia (P. incarnata L. × P. tucumanensis L.) were sterilized and placed in vitro. Direct shoot regeneration was achieved from young tendrils cultivated on MS medium containing, either 4.43 μM 6-benzylaminopurine (BAP) and 11.41 μM indoleacetic acid (IAA), or 49.20 μM 6-γ-γ-dimethylallylaminopurine (2iP) and 2.68 μM α–naphthalene acetic acid (NAA), respectively. In vitro shoot multiplication, rooting, and regenerated plant acclimatization protocols were established.

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Acknowledgments

The authors would like to thank Dr. Maurizio Vecchia for providing plant material and Mr Cesare Bianchini for technical help. This research was financed by the Italian Ministry of Agriculture and Forestry, Project 192/7303/2006 “Individuazione, caratterizzazione e valorizzazione di specie dotate di caratteristiche mediterranee (VIVAFLOR).” Publication no 7.

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Authors and Affiliations

  1. CRA-FSO Research Unit for Floriculture and Ornamental Species, Sanremo, Imperia, Italy

    Luca Pipino, Luca Braglia, Annalisa Giovannini & Antonio Mercuri

  2. CRA-SFM Research Unit for the Recovery and Improvement of Mediterranean Ornamental Plants S.S. 113-km 245,500, 1-90011, Bagheria, Palermo, Italy

    Giancarlo Fascella

Authors
  1. Luca Pipino
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  2. Luca Braglia
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  3. Annalisa Giovannini
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  4. Giancarlo Fascella
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  5. Antonio Mercuri
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Corresponding author

Correspondence to Antonio Mercuri .

Editor information

Editors and Affiliations

  1. Department of Applied Biology, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. UMRLEG, PCMV, INRA, Dijon CX, 21065, France

    Sergio J. Ochatt

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Pipino, L., Braglia, L., Giovannini, A., Fascella, G., Mercuri, A. (2010). In Vitro Regeneration and Multiplication of Passiflora Hybrid “Guglielmo Betto”. In: Jain, S., Ochatt, S. (eds) Protocols for In Vitro Propagation of Ornamental Plants. Methods in Molecular Biology, vol 589. Humana Press. https://doi.org/10.1007/978-1-60327-114-1_15

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  • DOI: https://doi.org/10.1007/978-1-60327-114-1_15

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-390-9

  • Online ISBN: 978-1-60327-114-1

  • eBook Packages: Springer Protocols

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