Abstract
Tissue culture techniques are routinely used for mass propagation and the establishment of disease free stock material. Virtually all pot type Anthuriums available in the market today are produced by tissue culture. In this chapter, we describe an efficient protocol to obtain Anthurium andreanum cv Rubrun vitro plants through micropropagation and organogenesis. Seeds from plant spadixes were germinated on MS medium supplemented with 0.5 mg/L BA. Micro-cuttings from in vitro germinated seedlings were subcultured on MS medium containing 2 mg/L BA and 0.5 mg/L NAA. Four-week-old in vitro plants obtained from microcuttings, showed callus proliferation at the stem base. The development of shoots and plantlets was observed from callus tissue. We also describe a detailed method for the histological analysis of callus tissue and a vitro plants acclimatization protocol.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kuehnle AR, Sugii N (1991) Callus induction and plantlet regeneration in tissue cultures of Hawaiian anthuriums. Hortsci 26:919–921
Pierik RLM, Steegmans HHM (1976) Vegetative propagation of Anthurium scherzerianum Schott through callus cultures. Scientia Hortic 4:291–292
Pierik RLM, Steegmans HHM, Van der Meys JAJ (1974) Plantlet formation in callus tissues of Anthurium andreanum Lind. Scientia Hortic 2:193–198
Vargas TE, Mejías A, Oropeza M, de García E (2004) Plant regeneration of Anthurium andreanum cv Rubrun. Electr J Biotech 7:282–286
Kunisaki JT (1980) In vitro propagation of Anthurium andreanum Lind. Hortsci 15:508–509
Chen FC, Kuehnle AR, Sugii N (1997) Anthurium roots for micropropagation and Agrobacterium tumefaciens-mediated gene transfer. Plant Cell Tiss Org Cult 49:71–74
Martin KP, Joseph D, Madassery J, Philip VJ (2003) Direct shoot regeneration from lamina explants of two commercial cut flower cultivars of Anthurium andreanum Hort. In Vitro Cell Dev Biol Plant 39:500–504
Teng WL (1997) Regeneration of anthurium adventitious shoots using liquid or raft culture. Plant Cell Tiss Org Cult 49:153–156
Geier T (1986) Factors affecting plant regeneration from leaf segments of Anthurium scherzerianum Schott cultured in vitro. Plant Cell Tiss Org Cult 6:115–125
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497
Acknowledgments
This research was supported by the Scientific and Humanistic Council of the “Universidad Central de Venezuela,” Caracas, Venezuela.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Maira, O., Alexander, M., Vargas, T.E. (2010). Micropropagation and Organogenesis of Anthurium andreanum Lind cv Rubrun. In: Jain, S., Ochatt, S. (eds) Protocols for In Vitro Propagation of Ornamental Plants. Methods in Molecular Biology, vol 589. Humana Press. https://doi.org/10.1007/978-1-60327-114-1_1
Download citation
DOI: https://doi.org/10.1007/978-1-60327-114-1_1
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-60327-390-9
Online ISBN: 978-1-60327-114-1
eBook Packages: Springer Protocols